9BAD

Crystal structure of Bacillus subtilis 168 L-asparaginase II with antileukemic activity

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	L-asparaginase 2	polymer	375	39965.6	4	UniProt (O34482) Pfam (PF00710) Pfam (PF17763)	Bacillus subtilis subsp. subtilis str. 168	L-ASNase 2,L-asparagine amidohydrolase 2
2	E, G (A, C)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
3	F (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	H, I, J, K (A, B, C, D)	water	water		18.0	1124	Chemie (HOH)

Sequence modifications

A, B, C, D: 23 - 375 (UniProt: O34482)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Leu 14	-	expression tag
Val 15	-	expression tag
Pro 16	-	expression tag
Arg 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
His 20	-	expression tag
Met 21	-	expression tag
Ala 22	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	159862.5
Non-Polymers*	Number of molecules	3
	Total formula weight	138.1
All*	Total formula weight	160000.6

*Water molecules are not included.