9B8V

AlphaFold2 informed cryo-EM model of the E. coli cellulose synthase BcsAG3B6 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F, G, H, I... (G, A, F, H, I...)	Cellulose synthase operon protein B	polymer	763	84376.0	6	UniProt (P37652) Pfam (PF03170)	Escherichia coli	Cellulose synthase regulatory subunit, BcsB
2	B, C, D (B, C, D)	Cellulose biosynthesis protein BcsG	polymer	567	63079.5	3	UniProt (P37659) Pfam (PF11658)	Escherichia coli
3	E (E)	Cellulose synthase catalytic subunit [UDP-forming]	polymer	887	101862.5	1	UniProt (P37653) Pfam (PF00535) Pfam (PF07238)	Escherichia coli	Bacterial cellulose synthase subunit A (BcsA)

Sequence modifications

G, A, F, H, I, J: 26 - 779 (UniProt: P37652)

PDB	External Database	Details
Ala 17	-	expression tag
Trp 18	-	expression tag
Ser 19	-	expression tag
His 20	-	expression tag
Pro 21	-	expression tag
Gln 22	-	expression tag
Phe 23	-	expression tag
Glu 24	-	expression tag
Lys 25	-	expression tag

B, C, D: 1 - 559 (UniProt: P37659)

PDB	External Database	Details
Asp 560	-	expression tag
Tyr 561	-	expression tag
Lys 562	-	expression tag
Asp 563	-	expression tag
Asp 564	-	expression tag
Asp 565	-	expression tag
Asp 566	-	expression tag
Lys 567	-	expression tag

E: 2 - 872 (UniProt: P37653)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 1	-	cloning artifact
His 873	-	expression tag
His 874	-	expression tag
His 875	-	expression tag
His 876	-	expression tag
His 877	-	expression tag
His 878	-	expression tag
Leu 879	-	expression tag
Glu 880	-	expression tag
His 881	-	expression tag
His 882	-	expression tag
His 883	-	expression tag
His 884	-	expression tag
His 885	-	expression tag
His 886	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	797357.0
All*	Total formula weight	797357.0

*Water molecules are not included.