9B6H

Cryo-EM structure of the mouse TRPM8 channel in complex with the antagonist TC-I 2014 and the cooling agonist C3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Transient receptor potential cation channel subfamily M member 8	polymer	1135	131548.3	4	UniProt (Q8R4D5) In PDB	Mus musculus (house mouse)	Long transient receptor potential channel 6,LTrpC-6,LTrpC6,Transient receptor potential p8,Trp-p8
2	A, B, C, D	3-{7-(trifluoromethyl)-5-[2-(trifluoromethyl)phenyl]-1H-benzimidazol-2-yl}-1-oxa-2-azaspiro[4.5]dec-2-ene	non-polymer		467.4	4	Chemie (T14)
3	A, B, C, D	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	12	Chemie (Y01)
4	D, A, B, C	nonyl(oxo)di(propan-2-yl)-lambda~5~-phosphane	non-polymer		260.4	4	Chemie (ULO)
5	D, A, B, C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)

Sequence modifications

A, B, C, D: 2 - 1104 (UniProt: Q8R4D5)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 1	-	expression tag
Ser 1105	-	expression tag
Asn 1106	-	expression tag
Ser 1107	-	expression tag
Leu 1108	-	expression tag
Glu 1109	-	expression tag
Val 1110	-	expression tag
Leu 1111	-	expression tag
Phe 1112	-	expression tag
Gln 1113	-	expression tag
Gly 1114	-	expression tag
Pro 1115	-	expression tag
Asp 1116	-	expression tag
Tyr 1117	-	expression tag
Lys 1118	-	expression tag
Asp 1119	-	expression tag
Asp 1120	-	expression tag
Asp 1121	-	expression tag
Asp 1122	-	expression tag
Lys 1123	-	expression tag
Ala 1124	-	expression tag
His 1125	-	expression tag
His 1126	-	expression tag
His 1127	-	expression tag
His 1128	-	expression tag
His 1129	-	expression tag
His 1130	-	expression tag
His 1131	-	expression tag
His 1132	-	expression tag
His 1133	-	expression tag
His 1134	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	526193.2
Non-Polymers*	Number of molecules	24
	Total formula weight	9636.8
All*	Total formula weight	535830.0

*Water molecules are not included.