9B5S
Ubiquitin E1-Ub-E2 tetrahedral transthiolation intermediate mimic (singly Ub-loaded) - cluster 4 map and model (ATP/Mg)
This is a non-PDB format compatible entry.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Ubiquitin-activating enzyme E1 1 | polymer | 1001 | 111764.0 | 1 | UniProt (O94609) Pfam (PF00899) Pfam (PF16190) Pfam (PF16191) Pfam (PF10585) Pfam (PF09358) In PDB | Schizosaccharomyces pombe 972h- | Poly(A)+ RNA transport protein 3 |
2 | C | Ubiquitin-conjugating enzyme E2 4 | polymer | 153 | 17043.3 | 1 | UniProt (P46595) Pfam (PF00179) In PDB | Schizosaccharomyces pombe 972h- | E2 ubiquitin-conjugating enzyme 4,Ubiquitin carrier protein 4,Ubiquitin-protein ligase 4 |
3 | D | Ubiquitin | polymer | 79 | 8769.9 | 1 | UniProt (P0CH07) Pfam (PF00240) In PDB | Schizosaccharomyces pombe 972h- | |
4 | A | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
5 | A | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
6 | D | 4-aminobutanenitrile | non-polymer | 84.1 | 1 | Chemie (A1AIV) |
Sequence modifications
A: 13 - 1012 (UniProt: O94609)
C: 1 - 147 (UniProt: P46595)
D: 79 - 153 (UniProt: P0CH07)
PDB | External Database | Details |
---|---|---|
Ser 12 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ser 21 | Cys 21 | engineered mutation |
Ser 107 | Cys 107 | engineered mutation |
Gly 148 | - | expression tag |
Gly 149 | - | expression tag |
Leu 150 | - | expression tag |
Val 151 | - | expression tag |
Pro 152 | - | expression tag |
Arg 153 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gly 75 | - | expression tag |
Ser 76 | - | expression tag |
Gly 77 | - | expression tag |
Gly 78 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 137577.3 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 639.9 | |
All* | Total formula weight | 138217.2 |