9B31

Cryo-EM structure of yeast (Nap1)2-Kap114-H2A-H2B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	KAP114 isoform 1	polymer	1010	114422.1	1	UniProt (A0A8H4BZV8) Pfam (PF03810) UniProt (by SIFTS) (P53067)	Saccharomyces cerevisiae (brewer's yeast)
2	B (B)	Histone H2A	polymer	131	13882.0	1	UniProt (A0A6A5Q402) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P04912)	Saccharomyces cerevisiae (brewer's yeast)
3	C (C)	Histone H2B	polymer	130	14133.1	1	UniProt (A0A6A5Q1U6) Pfam (PF00125) UniProt (by SIFTS) (P02294)	Saccharomyces cerevisiae (brewer's yeast)
4	D, E (D, E)	NAP1 isoform 1	polymer	313	36182.4	2	UniProt (A0A8H4BY55) Pfam (PF00956) UniProt (by SIFTS) (P25293)	Saccharomyces cerevisiae (brewer's yeast)

Sequence modifications

A: 1 - 1004 (UniProt: A0A8H4BZV8)

PDB	External Database	Details
Gly -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

D, E: 74 - 365 (UniProt: A0A8H4BY55)

PDB	External Database	Details
Met 53	-	initiating methionine
Gly 54	-	expression tag
Ser 55	-	expression tag
Ser 56	-	expression tag
His 57	-	expression tag
His 58	-	expression tag
His 59	-	expression tag
His 60	-	expression tag
His 61	-	expression tag
His 62	-	expression tag
Ser 63	-	expression tag
Ser 64	-	expression tag
Gly 65	-	expression tag
Leu 66	-	expression tag
Val 67	-	expression tag
Pro 68	-	expression tag
Arg 69	-	expression tag
Gly 70	-	expression tag
Ser 71	-	expression tag
His 72	-	expression tag
Met 73	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	214801.9
All*	Total formula weight	214801.9

*Water molecules are not included.