9B2N

Structure of the quorum quenching lactonase GcL D122N mutant - monometal center

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	quorum-quenching N-acyl-homoserine lactonase	polymer	297	34377.9	3	UniProt (A0A023DFE8) Pfam (PF00753)	Parageobacillus caldoxylosilyticus
2	D, I, U (A, B, C)	COBALT (II) ION	non-polymer		58.9	3	Chemie (CO)
3	E, F, G, J, K... (A, B, C)	SULFATE ION	non-polymer		96.1	12	Chemie (SO4)
4	H, Q, R, S (A, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	T, X (B, C)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
6	AA, Y, Z (C, A, B)	water	water		18.0	472	Chemie (HOH)

Sequence modifications

A, B, C: 2 - 283 (UniProt: A0A023DFE8)

PDB	External Database	Details
Trp -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
Pro -10	-	expression tag
Gln -9	-	expression tag
Phe -8	-	expression tag
Glu -7	-	expression tag
Lys -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Ser 1	-	expression tag
Asn 122	Asp 121	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	103133.8
Non-Polymers*	Number of molecules	21
	Total formula weight	1816.0
All*	Total formula weight	104949.8

*Water molecules are not included.