9B2I
Structure of the quorum quenching lactonase GcL G156P mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | quorum-quenching N-acyl-homoserine lactonase | polymer | 297 | 34419.0 | 3 | UniProt (A0A023DFE8) Pfam (PF00753) | Parageobacillus caldoxylosilyticus | GcL lactonase |
| 2 | D, I, IA, J, JA... (A, C, B) | ACETATE ION | non-polymer | 59.0 | 7 | Chemie (ACT) | |||
| 3 | E, FA, Q (A, C, B) | COBALT (II) ION | non-polymer | 58.9 | 3 | Chemie (CO) | |||
| 4 | F, G, GA, H, O... (A, C, B) | SULFATE ION | non-polymer | 96.1 | 11 | Chemie (SO4) | |||
| 5 | AA, BA, EA, K, KA... (B, C, A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 14 | Chemie (EDO) | |||
| 6 | CA, M, QA (B, A, C) | FE (III) ION | non-polymer | 55.8 | 3 | Chemie (FE) | |||
| 7 | DA, RA (B, C) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 2 | Chemie (PGE) | |||
| 8 | HA (C) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 9 | SA, TA, UA (A, B, C) | water | water | 18.0 | 358 | Chemie (HOH) |
Sequence modifications
A, B, C: 2 - 283 (UniProt: A0A023DFE8)
| PDB | External Database | Details |
|---|---|---|
| Trp -13 | - | expression tag |
| Ser -12 | - | expression tag |
| His -11 | - | expression tag |
| Pro -10 | - | expression tag |
| Gln -9 | - | expression tag |
| Phe -8 | - | expression tag |
| Glu -7 | - | expression tag |
| Lys -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Asn -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Tyr -2 | - | expression tag |
| Phe -1 | - | expression tag |
| Gln 0 | - | expression tag |
| Ser 1 | - | expression tag |
| Pro 156 | Gly 155 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 103256.9 | |
| Non-Polymers* | Number of molecules | 41 |
| Total formula weight | 3075.7 | |
| All* | Total formula weight | 106332.7 |
