9AVS
Human alpha-galactosidase A in complex with saposin B
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Alpha-galactosidase A | polymer | 414 | 47344.5 | 2 | UniProt (P06280) Pfam (PF16499) Pfam (PF17450) | Homo sapiens (human) | Alpha-D-galactosidase A,Alpha-D-galactoside galactohydrolase,Galactosylgalactosylglucosylceramidase GLA,Melibiase |
| 2 | C (C) | Saposin-B | polymer | 82 | 9097.5 | 1 | UniProt (P07602) Pfam (PF03489) | Homo sapiens (human) | Cerebroside sulfate activator,CSAct,Dispersin,Sphingolipid activator protein 1,SAP-1,Sulfatide/GM1 activator |
| 3 | D (E) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 894.8 | 1 | In PDB GlyTouCan (G42466VF) | |||
| 4 | E, G (D, G) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 2 | In PDB GlyTouCan (G81315DD) | |||
| 5 | F (F) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 6 | H (H) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 7 | I, J, K, L, M... (A, B) | SULFATE ION | non-polymer | 96.1 | 8 | Chemie (SO4) | |||
| 8 | Q (B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 9 | R, S (A, B) | water | water | 18.0 | 8 | Chemie (HOH) |
Sequence modifications
A, B: 32 - 429 (UniProt: P06280)
C: 1 - 79 (UniProt: P07602)
| PDB | External Database | Details |
|---|---|---|
| Ala 170 | Asp 170 | engineered mutation |
| Asp 430 | - | expression tag |
| Tyr 431 | - | expression tag |
| Lys 432 | - | expression tag |
| Asp 433 | - | expression tag |
| Asp 434 | - | expression tag |
| Asp 435 | - | expression tag |
| Asp 436 | - | expression tag |
| Lys 437 | - | expression tag |
| Asp 438 | - | expression tag |
| Tyr 439 | - | expression tag |
| Lys 440 | - | expression tag |
| Asp 441 | - | expression tag |
| Asp 442 | - | expression tag |
| Asp 443 | - | expression tag |
| Asp 444 | - | expression tag |
| Lys 445 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 103786.4 | |
| Branched | Number of molecules | 5 |
| Total formula weight | 3727.4 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 989.7 | |
| All* | Total formula weight | 108503.5 |
