9AU0

Cryo-EM structure of the BW245C-bound prostaglandin D2 receptor (DP1)-Gs complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37799.2	1	UniProt (P62873) Pfam (PF25391) UniProt (by SIFTS) (A0A8D2K0M5)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody-35	polymer	139	15271.9	1		Lama glama
5	E (R)	Prostaglandin D2 receptor	polymer	385	43222.9	1	UniProt (Q13258) Pfam (PF00001)	Homo sapiens (human)	PGD receptor,PGD2 receptor,Prostanoid DP receptor
6	F (R)	7-{(4S)-3-[(3R)-3-cyclohexyl-3-hydroxypropyl]-2,5-dioxoimidazolidin-4-yl}heptanoic acid	non-polymer		368.5	1	Chemie (A1AF8) PubChem (40481312) PubChem (18632066) PubChem (6603749) PubChem (3080928) PubChem (11451483) PubChem (12788338) PubChem (119304)
7	G (R)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
8	H (R)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	1	Chemie (Y01) PubChem (16219150) PubChem (22210610) PubChem (45489984) PubChem (60078761) PubChem (73440805) PubChem (99567469) PubChem (124301053) PubChem (124302591) PubChem (124303794) PubChem (124421343) PubChem (124427195) PubChem (124914595) PubChem (124914596) PubChem (131705526) PubChem (137332121) PubChem (137496357) PubChem (146157332) PubChem (154929912) PubChem (155199678) PubChem (156327905) PubChem (156426247) PubChem (156516588) PubChem (166979870) PubChem (167024614) PubChem (169132513) PubChem (170117245) PubChem (172400028) PubChem (57163942) PubChem (89891013) PubChem (99567468) PubChem (124427194) PubChem (124897021) PubChem (124897020) PubChem (10005657) PubChem (130340716) PubChem (11969853) PubChem (137392566) PubChem (137496323) PubChem (155804219) PubChem (169132514) PubChem (65082) PubChem (363416) PubChem (500055) PubChem (13301926) PubChem (13301927)
9	I, J, K, L, M (A, B, C, N, R)	water	water		18.0	139	Chemie (HOH)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gln 1	-	expression tag

R: 1 - 359 (UniProt: Q13258)

PDB	External Database	Details
Met -25	-	expression tag
Lys -24	-	expression tag
Thr -23	-	expression tag
Ile -22	-	expression tag
Ile -21	-	expression tag
Ala -20	-	expression tag
Leu -19	-	expression tag
Ser -18	-	expression tag
Tyr -17	-	expression tag
Ile -16	-	expression tag
Phe -15	-	expression tag
Cys -14	-	expression tag
Leu -13	-	expression tag
Val -12	-	expression tag
Phe -11	-	expression tag
Ala -10	-	expression tag
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	149838.7
Non-Polymers*	Number of molecules	3
	Total formula weight	1076.4
All*	Total formula weight	150915.1

*Water molecules are not included.