9ASI

Cryo-EM structure of the active Lactococcus lactis Csm bound to target in pre-cleavage stage

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	CRISPR-associated protein Csm4	polymer	297	33943.3	1	UniProt (L0CFH1) In PDB	Lactococcus lactis subsp. lactis
2	F, H, G, I	CRISPR system Cms endoribonuclease Csm3	polymer	214	23869.2	4	UniProt (L0CEA3) In PDB	Lactococcus lactis subsp. lactis	CRISPR type III A-associated RAMP protein Csm3
3	R	CRISPR RNA	polymer	37	11946.3	1		Lactococcus lactis subsp. lactis
4	D, E, C	CRISPR system Cms protein Csm2	polymer	150	17014.3	3	UniProt (L0CFW2) In PDB	Lactococcus lactis subsp. lactis	CRISPR type III A-associated protein Csm2
5	T	Target RNA	polymer	36	11558.9	1		Lactococcus lactis subsp. lactis
6	J	CRISPR system Cms protein Csm5	polymer	353	40621.8	1	UniProt (L0CG31) In PDB	Lactococcus lactis subsp. lactis	CRISPR type III A-associated protein Csm5
7	A	CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)	polymer	757	87223.7	1	UniProt (L0CEJ3) In PDB	Lactococcus lactis subsp. lactis	Cyclic oligoadenylate synthase
8	A	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
9	A	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
10	A	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)

Sequence modifications

D, E, C: 12 - 150 (UniProt: L0CFW2)

PDB	External Database	Details
Met 1	-	expression tag
Gly 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
Ser 9	-	expression tag
Gly 10	-	expression tag
Gly 11	-	expression tag

A: 1 - 757 (UniProt: L0CEJ3)

PDB	External Database	Details
Ala 13	His 13	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	331813.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1434.5
All*	Total formula weight	333248.1

*Water molecules are not included.