9ASI
Cryo-EM structure of the active Lactococcus lactis Csm bound to target in pre-cleavage stage
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | CRISPR-associated protein Csm4 | polymer | 297 | 33943.3 | 1 | UniProt (L0CFH1) Pfam (PF03787) Pfam (PF17953) | Lactococcus lactis subsp. lactis | |
| 2 | B, C, D, E (F, H, G, I) | CRISPR system Cms endoribonuclease Csm3 | polymer | 214 | 23869.2 | 4 | UniProt (L0CEA3) Pfam (PF03787) | Lactococcus lactis subsp. lactis | CRISPR type III A-associated RAMP protein Csm3 |
| 3 | F (R) | CRISPR RNA | polymer | 37 | 11946.3 | 1 | Lactococcus lactis subsp. lactis | ||
| 4 | G, H, J (D, E, C) | CRISPR system Cms protein Csm2 | polymer | 150 | 17014.3 | 3 | UniProt (L0CFW2) Pfam (PF03750) | Lactococcus lactis subsp. lactis | CRISPR type III A-associated protein Csm2 |
| 5 | I (T) | Target RNA | polymer | 36 | 11558.9 | 1 | Lactococcus lactis subsp. lactis | ||
| 6 | K (J) | CRISPR system Cms protein Csm5 | polymer | 353 | 40621.8 | 1 | UniProt (L0CG31) Pfam (PF03787) | Lactococcus lactis subsp. lactis | CRISPR type III A-associated protein Csm5 |
| 7 | L (A) | CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) | polymer | 757 | 87223.7 | 1 | UniProt (L0CEJ3) Pfam (PF01966) Pfam (PF18211) Pfam (PF22335) Pfam (PF20824) | Lactococcus lactis subsp. lactis | Cyclic oligoadenylate synthase |
| 8 | M, Q (A) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 9 | N, O, P (A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 10 | R (A) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) |
Sequence modifications
D, E, C: 12 - 150 (UniProt: L0CFW2)
A: 1 - 757 (UniProt: L0CEJ3)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| Gly 2 | - | expression tag |
| His 3 | - | expression tag |
| His 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| His 8 | - | expression tag |
| Ser 9 | - | expression tag |
| Gly 10 | - | expression tag |
| Gly 11 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 13 | His 13 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 331813.6 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1434.5 | |
| All* | Total formula weight | 333248.1 |
