9ARW

Structure of the guideless DtCmr Type III CRISPR complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (H)	CSD domain-containing protein Cmr6	polymer	376	42396.6	1	UniProt (A0A7G1H339) Pfam (PF00313) Pfam (PF03787)	Dissulfurispira thermophila
2	B (A)	Type III-B CRISPR-associated protein Cas10/Cmr2	polymer	612	70288.9	1	UniProt (A0A7G1H3Q2) Pfam (PF12469) Pfam (PF22335)	Dissulfurispira thermophila
3	C, D, E (C, D, E)	Type III-B CRISPR module RAMP protein Cmr4	polymer	315	34966.7	3	UniProt (A0A7G1H376) Pfam (PF03787)	Dissulfurispira thermophila
4	F, G (F, G)	CRISPR type III-B/RAMP module-associated protein Cmr5	polymer	140	15953.4	2	UniProt (A0A7G1H353) Pfam (PF09701)	Dissulfurispira thermophila
5	H (B)	Type III-B CRISPR module-associated protein Cmr3	polymer	360	40201.4	1	UniProt (A0A7G1H1A4) Pfam (PF09700)	Dissulfurispira thermophila
6	I (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 1 - 596 (UniProt: A0A7G1H3Q2)

PDB	External Database	Details
Met -15	-	initiating methionine
Gly -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	289693.6
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	289759.0

*Water molecules are not included.