9AR7
CryoEM structure of ThermoCas9 bound with target DNA strand only
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | single guide RNA | polymer | 149 | 47798.1 | 1 | Geobacillus thermodenitrificans | ||
| 2 | B (C) | cleaved 3' target DNA strand | polymer | 40 | 12238.9 | 1 | Geobacillus thermodenitrificans | ||
| 3 | C (P) | cleaved 5' target strand | polymer | 14 | 4310.8 | 1 | Geobacillus thermodenitrificans | ||
| 4 | D (A) | CRISPR-associated endonuclease Cas9 | polymer | 1082 | 126563.5 | 1 | UniProt (A0A1W6VMQ3) | Geobacillus thermodenitrificans | |
| 5 | E (B) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 6 | F, G, H, I (B, A) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) PubChem (888) | |||
| 7 | J, K, L (B, C, A) | water | water | 18.0 | 19 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 190911.2 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 620.4 | |
| All* | Total formula weight | 191531.6 |
