9AR7
CryoEM structure of ThermoCas9 bound with target DNA strand only
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | single guide RNA | polymer | 149 | 47798.1 | 1 | Geobacillus thermodenitrificans | ||
| 2 | B (C) | cleaved 3' target DNA strand | polymer | 40 | 12238.9 | 1 | Geobacillus thermodenitrificans | ||
| 3 | C (P) | cleaved 5' target strand | polymer | 14 | 4310.8 | 1 | Geobacillus thermodenitrificans | ||
| 4 | D (A) | CRISPR-associated endonuclease Cas9 | polymer | 1082 | 126563.5 | 1 | UniProt (A0A1W6VMQ3) | Geobacillus thermodenitrificans | |
| 5 | E (B) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 6 | F, G, H, I (B, A) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 7 | J, K, L (B, C, A) | water | water | 18.0 | 19 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 190911.2 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 620.4 | |
| All* | Total formula weight | 191531.6 |
