8ZYH
Crystal structure of a cupin protein (tm1459, I49C-4py/H52A/H54A/C106D mutant) in copper (Cu) substituted form
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Cupin type-2 domain-containing protein | polymer | 118 | 13295.2 | 2 | UniProt (Q9X1H0) Pfam (PF07883) | Thermotoga maritima MSB8 | |
| 2 | C (A) | HEXAETHYLENE GLYCOL | non-polymer | 282.3 | 1 | Chemie (P6G) | |||
| 3 | D, E, G (A, B) | COPPER (II) ION | non-polymer | 63.5 | 3 | Chemie (CU) | |||
| 4 | F, H (A, B) | 4-THIOPYRIDINE | non-polymer | 111.2 | 2 | Chemie (KN6) | |||
| 5 | I, J (A, B) | water | water | 18.0 | 213 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 114 (UniProt: Q9X1H0)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Cys 49 | Ile 49 | engineered mutation |
| Ala 52 | His 52 | engineered mutation |
| Ala 54 | His 54 | engineered mutation |
| Asp 106 | Cys 106 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 26590.3 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 695.3 | |
| All* | Total formula weight | 27285.6 |
