8ZYC

Cryo-EM structure of uropathogenic Escherichia coli CysK:CdiA:tRNA complex A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, E)	Cysteine synthase A	polymer	323	34756.7	2	UniProt (P0ABK6) Pfam (PF00291) UniProt (by SIFTS) (P0ABK5)	Escherichia coli	CSase A,O-acetylserine (thiol)-lyase A,OAS-TL A,O-acetylserine sulfhydrylase A,Sulfate starvation-induced protein 5,SSI5
2	B (B)	tRNA nuclease CdiA	polymer	234	25181.3	1	UniProt (Q0T963)	Escherichia coli 536	tRNase CdiA,Anticodon nuclease CdiA,CdiA-EC536,Toxin CdiA
3	C (C)	tRNAIleGAU	polymer	77	25000.9	1	GenBank (1845258627)	Escherichia coli
4	E, F (B, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

B: 1 - 227 (UniProt: Q0T963)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 178	His 3193	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	119695.5
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	119744.1

*Water molecules are not included.