8ZW0
Cryo-EM strcuture of Prostaglandin D2 Receptor DP1 activated by PGD2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45699.4 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (D) | Soluble cytochrome b562,Prostaglandin D2 receptor | polymer | 468 | 52055.9 | 1 | UniProt (P0ABE7) UniProt (Q13258) Pfam (PF00001) | Escherichia coli | Cytochrome b-562,PGD receptor,PGD2 receptor,Prostanoid DP receptor |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | E (N) | nanobody Nb35 | polymer | 156 | 16926.1 | 1 | Lama glama | ||
| 6 | F, G (D) | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) | |||
| 7 | H (D) | PROSTAGLANDIN D2 | non-polymer | 352.5 | 1 | Chemie (PG2) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
D: -113 - -7 (UniProt: P0ABE7)
D: 1 - 340 (UniProt: Q13258)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ala 268 | Glu 268 | engineered mutation |
| Lys 271 | Asn 271 | engineered mutation |
| Asp 274 | Lys 274 | engineered mutation |
| Lys 280 | Arg 280 | engineered mutation |
| Asp 284 | Thr 284 | engineered mutation |
| Thr 285 | Ile 285 | engineered mutation |
| Ser 366 | Ala 366 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -127 | - | initiating methionine |
| Lys -126 | - | expression tag |
| Thr -125 | - | expression tag |
| Ile -124 | - | expression tag |
| Ile -123 | - | expression tag |
| Ala -122 | - | expression tag |
| Leu -121 | - | expression tag |
| Ser -120 | - | expression tag |
| Tyr -119 | - | expression tag |
| Ile -118 | - | expression tag |
| Phe -117 | - | expression tag |
| Cys -116 | - | expression tag |
| Leu -115 | - | expression tag |
| Val -114 | - | expression tag |
| Trp -105 | Met 29 | conflict |
| Ile -10 | His 124 | conflict |
| Leu -6 | - | linker |
| Gly -5 | - | linker |
| Gly -4 | - | linker |
| Ser -3 | - | linker |
| Gly -2 | - | linker |
| Gly -1 | - | linker |
| Ser 0 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 160458.0 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1125.8 | |
| All* | Total formula weight | 161583.8 |
