8ZPT

Cryo-EM structure of prolactin-releasing peptide recognition with Gq

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(324) subunit alpha-1,	polymer	361	41708.4	1		Homo sapiens (human)
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	371	40227.0	1	UniProt (P62873) In PDB	Homo sapiens (human)
3	E	scfv16	polymer	247	26277.3	1		Homo sapiens (human)
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P59768) In PDB	Homo sapiens (human)	G gamma-I
5	L	Prolactin-releasing peptide PrRP20	polymer	21	2274.6	1	UniProt (P81277) In PDB	Homo sapiens (human)
6	R	Prolactin-releasing peptide receptor	polymer	370	41163.5	1	UniProt (P49683) In PDB	Homo sapiens (human)	PrRP receptor,PrRPR,G-protein coupled receptor 10,hGR3

Sequence modifications

B: 7 - 345 (UniProt: P62873)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gln 6	-	expression tag
Gly 346	-	expression tag
Ser 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Gly 355	-	expression tag
Gly 356	-	expression tag
Gly 357	-	expression tag
Ser 358	-	expression tag
Ser 359	-	expression tag
Gly 360	-	expression tag
Val 361	-	expression tag
Ser 362	-	expression tag
Gly 363	-	expression tag
Trp 364	-	expression tag
Arg 365	-	expression tag
Leu 366	-	expression tag
Phe 367	-	expression tag
Lys 368	-	expression tag
Lys 369	-	expression tag
Ile 370	-	expression tag
Ser 371	-	expression tag

L: 1 - 20 (UniProt: P81277)

PDB	External Database	Details
Nh2 21	-	amidation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	159380.7
All*	Total formula weight	159380.7

*Water molecules are not included.