8ZP7

Cryo-EM structure of Cas5-HNH Cascade bound with sDNA, Conf1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA (61-MER)	polymer	61	19697.7	1		Escherichia coli
2	B (C)	CRISPR-associated protein Cse1 (CRISPR_cse1)	polymer	546	62146.0	1	UniProt (A0A1V6F8D1) UniProt (by SIFTS) (A0ACD6BA32)	Candidatus Cloacimonetes bacterium ADurb.Bin088	Cas8
3	C (E)	CRISPR-associated protein Cse2 (CRISPR_cse2)	polymer	174	20300.6	1	UniProt (A0A1V6F8C9) UniProt (by SIFTS) (A0ACD6BA33)	Candidatus Cloacimonetes bacterium ADurb.Bin088	Cas11
4	D, E, F, G, H... (F, H, I, J, K...)	CRISPR system Cascade subunit CasC	polymer	378	41794.4	6	UniProt (A0A1V6F8B5) UniProt (by SIFTS) (A0ACD6BA34)	Candidatus Cloacimonetes bacterium ADurb.Bin088	Cas7
5	J (B)	CRISPR system Cascade subunit CasD	polymer	388	43621.4	1	UniProt (A0A1V6F8C5) UniProt (by SIFTS) (A0ACD6BA35)	Candidatus Cloacimonetes bacterium ADurb.Bin088	Cas5
6	K (D)	CRISPR-associated endoribonuclease Cse3	polymer	272	31218.8	1	UniProt (A0A1V6F8C4) UniProt (by SIFTS) (A0ACD6BA36)	Candidatus Cloacimonetes bacterium ADurb.Bin088	Cas6
7	L (M)	DNA (60-MER)	polymer	60	18427.8	1		Escherichia coli
8	M, N (C, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

C: 2 - 535 (UniProt: A0A1V6F8D1)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Val 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	446178.5
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	446227.1

*Water molecules are not included.