8ZMZ

Cryo-EM structure of R-eLACCO2 in the lactate-bound state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Lactate-binding periplasmic protein TTHA0766,Red fluorescent protein drFP583	polymer	622	70044.5	1	UniProt (Q5SK82) UniProt (Q9U6Y8) Pfam (PF03480) Pfam (PF01353)	Thermus thermophilus HB8	ABC transporter,solute-binding protein,Extracytoplasmic solute receptor protein TTHA0766,TRAP transporter lactate-binding subunit P,DsRed
2	B (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
3	C (A)	(2S)-2-HYDROXYPROPANOIC ACID	non-polymer		90.1	1	Chemie (2OP)

Sequence modifications

A: 2 - 191 (UniProt: Q5SK82)

PDB	External Database	Details
Met -35	-	initiating methionine
Gly -34	-	expression tag
Gly -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Gly -25	-	expression tag
Met -24	-	expression tag
Ala -23	-	expression tag
Ser -22	-	expression tag
Met -21	-	expression tag
Thr -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Gln -17	-	expression tag
Gln -16	-	expression tag
Met -15	-	expression tag
Gly -14	-	expression tag
Arg -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Tyr -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Pro -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Arg 0	-	expression tag
Met 1	-	expression tag
Met 5	Leu 26	engineered mutation
Lys 14	Arg 35	engineered mutation
Leu 51	Phe 72	engineered mutation
Glu 83	Gly 104	engineered mutation
Asn 116	Ile 137	engineered mutation
Thr 138	Ile 159	engineered mutation
Ala 190	Val 211	engineered mutation
Val 191	Ile 212	engineered mutation

A: 194 - 269 (UniProt: Q9U6Y8)

PDB	External Database	Details
Val 194	Ser 146	engineered mutation
Ser 195	Thr 147	engineered mutation
Met 198	Leu 150	engineered mutation
Glu 201	Arg 153	engineered mutation
Ala 204	Val 156	engineered mutation
Ser 207	Gly 159	engineered mutation
Lys 210	His 162	engineered mutation
Gly 212	Ala 164	engineered mutation
Arg 214	Lys 166	engineered mutation
Ala 222	Leu 174	engineered mutation
Ala 223	Val 175	engineered mutation
Val 224	Glu 176	engineered mutation
Val 225	Phe 177	engineered mutation
Thr 227	Ser 179	engineered mutation
Thr 228	Ile 180	engineered mutation
Lys 230	Met 182	engineered mutation
Ala 240	Tyr 192	engineered mutation
Val 242	Tyr 194	engineered mutation
Ile 245	Ser 197	engineered mutation
Gln 246	Lys 198	engineered mutation
Val 250	Thr 202	engineered mutation
Cys 262	Tyr 214	engineered mutation
Ala 265	Thr 217	engineered mutation

A: 298 - 433 (UniProt: Q9U6Y8)

PDB	External Database	Details
Ser 270	-	linker
Thr 271	-	linker
Gly 272	-	linker
Gly 273	-	linker
Val 274	-	linker
Val 275	-	linker
Glu 276	-	linker
Leu 277	-	linker
Asp 278	-	linker
Lys 279	-	linker
Gly 280	-	linker
Gly 281	-	linker
Thr 282	-	linker
Gly 283	-	linker
Gly 284	-	linker
Ser 285	-	linker
Leu 286	-	linker
Val 287	-	linker
Ser 288	-	linker
Lys 289	-	linker
Gly 290	-	linker
Glu 291	-	linker
Glu 292	-	linker
Asp 293	-	linker
Asp 294	-	linker
Met 295	-	linker
Ala 296	-	linker
Ile 297	-	linker
Val 298	Ile 8	engineered mutation
His 307	Arg 17	engineered mutation
Ser 311	Thr 21	engineered mutation
Ala 330	Gly 40	engineered mutation
Phe 331	His 41	engineered mutation
Gln 332	Asn 42	engineered mutation
Ala 334	Val 44	engineered mutation
Nrq 357	Gln 66	chromophore
Nrq 357	Tyr 67	chromophore
Nrq 357	Gly 68	chromophore
Ala 361	Val 71	engineered mutation
Ile 363	Val 73	engineered mutation
Phe 373	Lys 83	engineered mutation
Arg 382	Lys 92	engineered mutation
Tyr 388	Asn 98	engineered mutation
Ile 394	Val 104	engineered mutation
Ile 395	Val 105	engineered mutation
His 396	Thr 106	engineered mutation
Asp 398	Thr 108	engineered mutation
Val 407	Cys 117	engineered mutation
Leu 414	Phe 124	engineered mutation
Arg 415	Ile 125	engineered mutation
Thr 417	Val 127	engineered mutation
Pro 421	Ser 131	engineered mutation
Glu 434	-	linker
Arg 435	-	linker
Gly 436	-	linker
Arg 437	-	linker

A: 438 - 586 (UniProt: Q5SK82)

PDB	External Database	Details
Thr 192	-	linker
Ala 193	-	linker
Ser 438	Asp 213	engineered mutation
Glu 456	Val 231	engineered mutation
Gly 534	Ser 309	engineered mutation
Arg 571	Lys 346	engineered mutation
His 575	Tyr 350	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	70044.5
Non-Polymers*	Number of molecules	2
	Total formula weight	130.2
All*	Total formula weight	70174.7

*Water molecules are not included.