8ZJY

Cryo-EM structure of pAg-bound BTN2A1-BTN3A1-BTN3A3 in complex with Vgamma9-Vdelta2 TCR (G115 genotype)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Butyrophilin subfamily 2 member A1	polymer	534	60684.5	2	UniProt (Q7KYR7) Pfam (PF07686) Pfam (PF22705) Pfam (PF13765) Pfam (PF00622)	Homo sapiens (human)
2	C (D)	T cell receptor delta variable 2, T cell receptor delta constant 1	polymer	204	22746.9	1		Homo sapiens
3	D (E)	Butyrophilin subfamily 3 member A3	polymer	589	65729.4	1	UniProt (O00478) Pfam (PF07686) Pfam (PF22705) Pfam (PF13765) Pfam (PF00622)	Homo sapiens (human)
4	E (F)	Butyrophilin subfamily 3 member A1	polymer	484	54428.8	1	UniProt (O00481) Pfam (PF22705) Pfam (PF13765) Pfam (PF00622) Pfam (PF07686)	Homo sapiens (human)
5	F (G)	T cell receptor gamma variable 9, T cell receptor gamma constant 1	polymer	229	25927.6	1		Homo sapiens
6	G (F)	(2E)-4-hydroxy-3-methylbut-2-en-1-yl trihydrogen diphosphate	non-polymer		262.1	1	Chemie (H6P)

Sequence modifications

A, B: 29 - 527 (UniProt: Q7KYR7)

PDB	External Database	Details
Met 7	-	initiating methionine
Asp 8	-	expression tag
Met 9	-	expression tag
Arg 10	-	expression tag
Val 11	-	expression tag
Pro 12	-	expression tag
Ala 13	-	expression tag
Gln 14	-	expression tag
Leu 15	-	expression tag
Leu 16	-	expression tag
Gly 17	-	expression tag
Leu 18	-	expression tag
Leu 19	-	expression tag
Leu 20	-	expression tag
Leu 21	-	expression tag
Trp 22	-	expression tag
Leu 23	-	expression tag
Ser 24	-	expression tag
Gly 25	-	expression tag
Ala 26	-	expression tag
Arg 27	-	expression tag
Cys 28	-	expression tag
Gly 528	-	expression tag
Ser 529	-	expression tag
Ser 530	-	expression tag
Gly 531	-	expression tag
Met 532	-	expression tag
Asp 533	-	expression tag
Tyr 534	-	expression tag
Lys 535	-	expression tag
Asp 536	-	expression tag
Asp 537	-	expression tag
Asp 538	-	expression tag
Asp 539	-	expression tag
Lys 540	-	expression tag

E: 30 - 584 (UniProt: O00478)

PDB	External Database	Details
Met 8	-	initiating methionine
Asp 9	-	expression tag
Met 10	-	expression tag
Arg 11	-	expression tag
Val 12	-	expression tag
Pro 13	-	expression tag
Ala 14	-	expression tag
Gln 15	-	expression tag
Leu 16	-	expression tag
Leu 17	-	expression tag
Gly 18	-	expression tag
Leu 19	-	expression tag
Leu 20	-	expression tag
Leu 21	-	expression tag
Leu 22	-	expression tag
Trp 23	-	expression tag
Leu 24	-	expression tag
Ser 25	-	expression tag
Gly 26	-	expression tag
Ala 27	-	expression tag
Arg 28	-	expression tag
Cys 29	-	expression tag
Gly 585	-	expression tag
Ser 586	-	expression tag
Ser 587	-	expression tag
Gly 588	-	expression tag
His 589	-	expression tag
His 590	-	expression tag
His 591	-	expression tag
His 592	-	expression tag
His 593	-	expression tag
His 594	-	expression tag
His 595	-	expression tag
His 596	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	290201.6
Non-Polymers*	Number of molecules	1
	Total formula weight	262.1
All*	Total formula weight	290463.7

*Water molecules are not included.