8ZIW

enteropeptidase with E574A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Enteropeptidase non-catalytic heavy chain	polymer	284	31077.9	1	UniProt (P98073) Pfam (PF00431) Pfam (PF00057) Pfam (PF15494)	Homo sapiens (human)
2	B (D)	Enteropeptidase catalytic light chain	polymer	235	26150.7	1	UniProt (P98073) Pfam (PF00089)	Homo sapiens (human)
3	C, H (B, H)	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	2	In PDB GlyTouCan (G22573RC)
4	D, F, J, L (C, F, J, L)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	4	In PDB GlyTouCan (G15407YE)
5	E, I (E, I)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	2	In PDB GlyTouCan (G81315DD)
6	G (G)	beta-D-mannopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G73474XW)
7	K (K)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G62916OK)

Sequence modifications

A: 501 - 784 (UniProt: P98073)

PDB	External Database	Details
Ala 574	Glu 574	engineered mutation

D: 785 - 1019 (UniProt: P98073)

PDB	External Database	Details
Ala 825	His 825	engineered mutation
Ala 876	Asp 876	engineered mutation
Ala 971	Ser 971	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57228.6
Branched	Number of molecules	10
	Total formula weight	6514.0
All*	Total formula weight	63742.6

*Water molecules are not included.