8ZGG

Human lysine O-link glycosylation complex, LH3/ColGalT1 with bound UDP-glucose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3	polymer	778	89776.0	4	UniProt (O60568) Pfam (PF25342) Pfam (PF25238) Pfam (PF03171)	Homo sapiens (human)
2	E, F, G, H (U, V, W, X)	Procollagen galactosyltransferase 1	polymer	653	74976.4	4	UniProt (Q8NBJ5) Pfam (PF13704) Pfam (PF01755)	Homo sapiens (human)	Collagen beta(1-O)galactosyltransferase 1,ColGalT 1,Glycosyltransferase 25 family member 1,Hydroxylysine galactosyltransferase 1
3	I, J (E, F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	K, P, Q (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
5	L, R (A, B)	2-OXOGLUTARIC ACID	non-polymer		146.1	2	Chemie (AKG) PubChem (51)
6	M, S (A, B)	FE (II) ION	non-polymer		55.8	2	Chemie (FE2) PubChem (27284)
7	N, T (A, B)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	2	Chemie (UDP) PubChem (25323044) PubChem (25323048) PubChem (44298965) PubChem (53486216) PubChem (57448939) PubChem (60098857) PubChem (1158) PubChem (6031) PubChem (91746160) PubChem (92332560) PubChem (92332561) PubChem (92332562) PubChem (92332563) PubChem (93088131) PubChem (93630083) PubChem (3084486) PubChem (6101632) PubChem (6604175) PubChem (11811538) PubChem (12049241)
8	O, U, W, Y (A, B, U, V)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN) PubChem (27854)
9	V, X (U, V)	[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-[[(2~{S},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]methyl]phosphinic acid	non-polymer		564.3	2	Chemie (660)

Sequence modifications

A, B, C, D: 1 - 738 (UniProt: O60568)

PDB	External Database	Details
Ala 739	-	expression tag
Ala 740	-	expression tag
Ala 741	-	expression tag
Glu 742	-	expression tag
Asn 743	-	expression tag
Leu 744	-	expression tag
Tyr 745	-	expression tag
Phe 746	-	expression tag
Gln 747	-	expression tag
Gly 748	-	expression tag
Asp 749	-	expression tag
Tyr 750	-	expression tag
Lys 751	-	expression tag
Asp 752	-	expression tag
His 753	-	expression tag
Asp 754	-	expression tag
Gly 755	-	expression tag
Asp 756	-	expression tag
Tyr 757	-	expression tag
Lys 758	-	expression tag
Asp 759	-	expression tag
His 760	-	expression tag
Asp 761	-	expression tag
Ile 762	-	expression tag
Asp 763	-	expression tag
Tyr 764	-	expression tag
Lys 765	-	expression tag
Asp 766	-	expression tag
Asp 767	-	expression tag
Asp 768	-	expression tag
Asp 769	-	expression tag
Lys 770	-	expression tag
His 771	-	expression tag
His 772	-	expression tag
His 773	-	expression tag
His 774	-	expression tag
His 775	-	expression tag
His 776	-	expression tag
His 777	-	expression tag
His 778	-	expression tag

U, V, W, X: 30 - 622 (UniProt: Q8NBJ5)

PDB	External Database	Details
Met -27	-	initiating methionine
Lys -26	-	expression tag
Thr -25	-	expression tag
Ile -24	-	expression tag
Ile -23	-	expression tag
Ala -22	-	expression tag
Leu -21	-	expression tag
Ser -20	-	expression tag
Tyr -19	-	expression tag
Ile -18	-	expression tag
Phe -17	-	expression tag
Cys -16	-	expression tag
Leu -15	-	expression tag
Val -14	-	expression tag
Phe -13	-	expression tag
Ala -12	-	expression tag
Trp -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
Pro -8	-	expression tag
Gln -7	-	expression tag
Phe -6	-	expression tag
Glu -5	-	expression tag
Lys -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Gly 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
Gly 5	-	expression tag
Gly 6	-	expression tag
Ser 7	-	expression tag
Ala 8	-	expression tag
Trp 9	-	expression tag
Ser 10	-	expression tag
His 11	-	expression tag
Pro 12	-	expression tag
Gln 13	-	expression tag
Phe 14	-	expression tag
Glu 15	-	expression tag
Lys 16	-	expression tag
Ser 17	-	expression tag
Ala 18	-	expression tag
Leu 19	-	expression tag
Glu 20	-	expression tag
Val 21	-	expression tag
Leu 22	-	expression tag
Phe 23	-	expression tag
Gln 24	-	expression tag
Gly 25	-	expression tag
Pro 26	-	expression tag
Gly 27	-	expression tag
Arg 28	-	expression tag
Ala 29	-	expression tag
Ala 623	-	expression tag
Ala 624	-	expression tag
Ala 625	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	659009.9
Branched	Number of molecules	2
	Total formula weight	848.8
Non-Polymers*	Number of molecules	15
	Total formula weight	3224.2
All*	Total formula weight	663082.9

*Water molecules are not included.