8ZGC

Human lysine O-link glycosylation complex, LH3/ColGalT1 with bound UDP-galactose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F (A, B, C, D)	Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3	polymer	778	89776.0	4	UniProt (O60568) Pfam (PF03171)	Homo sapiens (human)
2	C, D, G, H (U, V, W, X)	Procollagen galactosyltransferase 1	polymer	653	74976.4	4	UniProt (Q8NBJ5) Pfam (PF13704) Pfam (PF01755)	Homo sapiens (human)	Collagen beta(1-O)galactosyltransferase 1,ColGalT 1,Glycosyltransferase 25 family member 1,Hydroxylysine galactosyltransferase 1
3	I, J (E, F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	K, L, Q, R (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
5	M, S (A, B)	2-OXOGLUTARIC ACID	non-polymer		146.1	2	Chemie (AKG)
6	N, T (A, B)	FE (II) ION	non-polymer		55.8	2	Chemie (FE2)
7	BA, O, U, X (V, A, B, U)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	4	Chemie (UDP)
8	CA, DA, P, V, Y... (V, A, B, U)	MANGANESE (II) ION	non-polymer		54.9	6	Chemie (MN)
9	AA, W (V, U)	GALACTOSE-URIDINE-5'-DIPHOSPHATE	non-polymer		566.3	2	Chemie (GDU)

Sequence modifications

A, B, C, D: 1 - 738 (UniProt: O60568)

PDB	External Database	Details
Ala 739	-	expression tag
Ala 740	-	expression tag
Ala 741	-	expression tag
Glu 742	-	expression tag
Asn 743	-	expression tag
Leu 744	-	expression tag
Tyr 745	-	expression tag
Phe 746	-	expression tag
Gln 747	-	expression tag
Gly 748	-	expression tag
Asp 749	-	expression tag
Tyr 750	-	expression tag
Lys 751	-	expression tag
Asp 752	-	expression tag
His 753	-	expression tag
Asp 754	-	expression tag
Gly 755	-	expression tag
Asp 756	-	expression tag
Tyr 757	-	expression tag
Lys 758	-	expression tag
Asp 759	-	expression tag
His 760	-	expression tag
Asp 761	-	expression tag
Ile 762	-	expression tag
Asp 763	-	expression tag
Tyr 764	-	expression tag
Lys 765	-	expression tag
Asp 766	-	expression tag
Asp 767	-	expression tag
Asp 768	-	expression tag
Asp 769	-	expression tag
Lys 770	-	expression tag
His 771	-	expression tag
His 772	-	expression tag
His 773	-	expression tag
His 774	-	expression tag
His 775	-	expression tag
His 776	-	expression tag
His 777	-	expression tag
His 778	-	expression tag

U, V, W, X: 30 - 622 (UniProt: Q8NBJ5)

PDB	External Database	Details
Met -27	-	initiating methionine
Lys -26	-	expression tag
Thr -25	-	expression tag
Ile -24	-	expression tag
Ile -23	-	expression tag
Ala -22	-	expression tag
Leu -21	-	expression tag
Ser -20	-	expression tag
Tyr -19	-	expression tag
Ile -18	-	expression tag
Phe -17	-	expression tag
Cys -16	-	expression tag
Leu -15	-	expression tag
Val -14	-	expression tag
Phe -13	-	expression tag
Ala -12	-	expression tag
Trp -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
Pro -8	-	expression tag
Gln -7	-	expression tag
Phe -6	-	expression tag
Glu -5	-	expression tag
Lys -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Gly 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
Gly 5	-	expression tag
Gly 6	-	expression tag
Ser 7	-	expression tag
Ala 8	-	expression tag
Trp 9	-	expression tag
Ser 10	-	expression tag
His 11	-	expression tag
Pro 12	-	expression tag
Gln 13	-	expression tag
Phe 14	-	expression tag
Glu 15	-	expression tag
Lys 16	-	expression tag
Ser 17	-	expression tag
Ala 18	-	expression tag
Leu 19	-	expression tag
Glu 20	-	expression tag
Val 21	-	expression tag
Leu 22	-	expression tag
Phe 23	-	expression tag
Gln 24	-	expression tag
Gly 25	-	expression tag
Pro 26	-	expression tag
Gly 27	-	expression tag
Arg 28	-	expression tag
Ala 29	-	expression tag
Ala 623	-	expression tag
Ala 624	-	expression tag
Ala 625	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	659009.9
Branched	Number of molecules	2
	Total formula weight	848.8
Non-Polymers*	Number of molecules	20
	Total formula weight	4367.6
All*	Total formula weight	664226.3

*Water molecules are not included.