8ZER

Crystal structure of the complex of Wuhan SARS-CoV-2 RBD (319-541) with P2C5 nanobody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, C, E, G, I	Spike protein S1	polymer	235	26647.9	5	UniProt (P0DTC2) In PDB	Severe acute respiratory syndrome coronavirus 2
2	A, D, F, H, J	Nanobody P2C5	polymer	146	16046.5	5		Camelus bactrianus
3	K, N	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
4	L, M	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
5	O	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-beta-D-mannopyranose-(1-4)-[beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1276.2	1	In PDB

Sequence modifications

B, C, E, G, I: 319 - 541 (UniProt: P0DTC2)

PDB	External Database	Details
Gly 542	-	expression tag
Ser 543	-	expression tag
His 544	-	expression tag
His 545	-	expression tag
His 546	-	expression tag
His 547	-	expression tag
His 548	-	expression tag
His 549	-	expression tag
His 550	-	expression tag
His 551	-	expression tag
His 552	-	expression tag
His 553	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	213472.4
Branched	Number of molecules	5
	Total formula weight	3298.0
All*	Total formula weight	216770.4

*Water molecules are not included.