8ZD1
Cryo-EM structure of the xGPR4-Gs complex in pH6.2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 374 | 43432.9 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 338 | 37198.7 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 52 | 5817.7 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (R) | G-protein coupled receptor 4 | polymer | 289 | 31365.9 | 1 | UniProt (A0A6I8PUB9) Pfam (PF00001) | Xenopus tropicalis (tropical clawed frog) | |
| 5 | E (S) | scFv16 | polymer | 250 | 26678.7 | 1 | synthetic construct |
Sequence modifications
A: 26 - 394 (UniProt: P63092)
R: 13 - 301 (UniProt: A0A6I8PUB9)
| PDB | External Database | Details |
|---|---|---|
| Thr 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Ser 6 | - | expression tag |
| Ala 7 | - | expression tag |
| Glu 8 | - | expression tag |
| Asp 9 | - | expression tag |
| Lys 10 | - | expression tag |
| Ala 11 | - | expression tag |
| Ala 12 | - | expression tag |
| Val 13 | - | expression tag |
| Glu 14 | - | expression tag |
| Arg 15 | - | expression tag |
| Ser 16 | - | expression tag |
| Lys 17 | - | expression tag |
| Met 18 | - | expression tag |
| Asp 49 | Gly 49 | conflict |
| Asn 50 | Glu 50 | conflict |
| Tyr 63 | Leu 63 | conflict |
| Lys 213 | Leu 203 | conflict |
| Ala 236 | Gly 226 | conflict |
| Asp 259 | Ala 249 | conflict |
| Asp 262 | Ser 252 | conflict |
| - | Asn 254 | deletion |
| - | Met 255 | deletion |
| - | Val 256 | deletion |
| - | Ile 257 | deletion |
| - | Arg 258 | deletion |
| - | Glu 259 | deletion |
| - | Asp 260 | deletion |
| - | Asn 261 | deletion |
| - | Gln 262 | deletion |
| - | Thr 263 | deletion |
| Ala 264 | Asn 264 | conflict |
| Asp 272 | Leu 272 | conflict |
| Ser 366 | Ala 366 | conflict |
| Ala 372 | Ile 372 | conflict |
| Ile 375 | Val 375 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 155 | Gly 155 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 144493.8 | |
| All* | Total formula weight | 144493.8 |
