8ZBE

cryo-EM structure of the octreotide-bound SSTR5-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-2 adrenergic receptor,Somatostatin receptor type 5,lgbit (fusion protein)	polymer	570	62015.9	1	UniProt (P07550) UniProt (P35346) Pfam (PF00001)	Homo sapiens (human)	Beta-2 adrenoreceptor,Beta-2 adrenoceptor,SS-5-R,SS5-R,SS5R,SST5
2	B (B)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C (D)	Guanine nucleotide-binding protein subunit gamma	polymer	71	7861.1	1	UniProt (A0A9X9ZA26) Pfam (PF00631)	Rattus norvegicus (Norway rat)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
4	D (E)	ScFv16	polymer	304	32708.5	1		Homo sapiens (human)
5	E (P)	octreotide	polymer	8	1036.2	1		synthetic construct
6	F (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	377	41055.9	1	UniProt (P54311) Pfam (PF00400)	Rattus norvegicus (Norway rat)	Transducin beta chain 1

Sequence modifications

A: -23 - 0 (UniProt: P07550)

PDB	External Database	Details
Met -47	-	initiating methionine
Lys -46	-	expression tag
Thr -45	-	expression tag
Ile -44	-	expression tag
Ile -43	-	expression tag
Ala -42	-	expression tag
Leu -41	-	expression tag
Ser -40	-	expression tag
Tyr -39	-	expression tag
Ile -38	-	expression tag
Phe -37	-	expression tag
Cys -36	-	expression tag
Leu -35	-	expression tag
Val -34	-	expression tag
Phe -33	-	expression tag
Ala -32	-	expression tag
Asp -31	-	expression tag
Tyr -30	-	expression tag
Lys -29	-	expression tag
Asp -28	-	expression tag
Asp -27	-	expression tag
Asp -26	-	expression tag
Asp -25	-	expression tag
Lys -24	-	expression tag

A: 1 - 364 (UniProt: P35346)
A: 365 - 522 (PDB: 8ZBE)
B: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	conflict
Ser 326	Ala 326	conflict

C: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	185122.7
All*	Total formula weight	185122.7

*Water molecules are not included.