8ZBE
cryo-EM structure of the octreotide-bound SSTR5-Gi complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Beta-2 adrenergic receptor,Somatostatin receptor type 5,lgbit (fusion protein) | polymer | 570 | 62015.9 | 1 | UniProt (P07550) UniProt (P35346) Pfam (PF00001) In PDB | Homo sapiens (human) | Beta-2 adrenoreceptor,Beta-2 adrenoceptor,SS-5-R,SS5-R,SS5R,SST5 |
2 | B | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40445.1 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
3 | D | Guanine nucleotide-binding protein subunit gamma | polymer | 71 | 7861.1 | 1 | UniProt (A0A9X9ZA26) Pfam (PF00631) In PDB | Rattus norvegicus (Norway rat) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 |
4 | E | ScFv16 | polymer | 304 | 32708.5 | 1 | Homo sapiens (human) | ||
5 | P | octreotide | polymer | 8 | 1036.2 | 1 | synthetic construct | ||
6 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 377 | 41055.9 | 1 | UniProt (P54311) Pfam (PF00400) In PDB | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
Sequence modifications
A: -23 - 0 (UniProt: P07550)
A: 1 - 364 (UniProt: P35346)
A: 365 - 522 (PDB: 8ZBE)
B: 1 - 354 (UniProt: P63096)
C: 2 - 340 (UniProt: P54311)
PDB | External Database | Details |
---|---|---|
Met -47 | - | initiating methionine |
Lys -46 | - | expression tag |
Thr -45 | - | expression tag |
Ile -44 | - | expression tag |
Ile -43 | - | expression tag |
Ala -42 | - | expression tag |
Leu -41 | - | expression tag |
Ser -40 | - | expression tag |
Tyr -39 | - | expression tag |
Ile -38 | - | expression tag |
Phe -37 | - | expression tag |
Cys -36 | - | expression tag |
Leu -35 | - | expression tag |
Val -34 | - | expression tag |
Phe -33 | - | expression tag |
Ala -32 | - | expression tag |
Asp -31 | - | expression tag |
Tyr -30 | - | expression tag |
Lys -29 | - | expression tag |
Asp -28 | - | expression tag |
Asp -27 | - | expression tag |
Asp -26 | - | expression tag |
Asp -25 | - | expression tag |
Lys -24 | - | expression tag |
A: 365 - 522 (PDB: 8ZBE)
B: 1 - 354 (UniProt: P63096)
PDB | External Database | Details |
---|---|---|
Ala 203 | Gly 203 | conflict |
Ser 326 | Ala 326 | conflict |
PDB | External Database | Details |
---|---|---|
Met -10 | - | initiating methionine |
His -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Leu -1 | - | expression tag |
Leu 0 | - | expression tag |
Gln 1 | - | expression tag |
Gly 341 | - | expression tag |
Ser 342 | - | expression tag |
Ser 343 | - | expression tag |
Gly 344 | - | expression tag |
Gly 345 | - | expression tag |
Gly 346 | - | expression tag |
Gly 347 | - | expression tag |
Ser 348 | - | expression tag |
Gly 349 | - | expression tag |
Gly 350 | - | expression tag |
Gly 351 | - | expression tag |
Gly 352 | - | expression tag |
Ser 353 | - | expression tag |
Ser 354 | - | expression tag |
Gly 355 | - | expression tag |
Val 356 | - | expression tag |
Ser 357 | - | expression tag |
Gly 358 | - | expression tag |
Trp 359 | - | expression tag |
Arg 360 | - | expression tag |
Leu 361 | - | expression tag |
Phe 362 | - | expression tag |
Lys 363 | - | expression tag |
Lys 364 | - | expression tag |
Ile 365 | - | expression tag |
Ser 366 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 185122.7 | |
All* | Total formula weight | 185122.7 |