8ZBB

Cryo-EM structure of outward state Anhydromuropeptide permease (AmpG) G50W/L269W

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Muropeptide transporter,Soluble cytochrome b562	polymer	582	63770.9	1	UniProt (A0AB38FS76) UniProt (P0ABE7)	Yokenella regensburgei	Cytochrome b-562
2	B (H)	anti-BRIL Fab Heavy chain	polymer	225	23904.7	1		Homo sapiens
3	C (K)	anti-BRIL Fab Nanobody	polymer	120	13159.4	1		synthetic construct
4	D (L)	anti-BRIL Fab Light chain	polymer	212	23209.8	1		Homo sapiens

Sequence modifications

A: 12 - 198 (UniProt: A0AB38FS76)

PDB	External Database	Details
Trp 50	Gly 50	engineered mutation

A: 199 - 303 (UniProt: P0ABE7)

PDB	External Database	Details
Ala 202	Glu 26	engineered mutation
Trp 205	Met 29	engineered mutation
Ile 300	His 124	engineered mutation
Leu 304	-	linker
Gly 305	-	linker

A: 306 - 593 (UniProt: A0AB38FS76)

PDB	External Database	Details
Trp 372	Leu 269	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	124044.8
All*	Total formula weight	124044.8

*Water molecules are not included.