8Z90

Cryo-EM structure of Thogoto virus polymerase in transcription initiation conformation 2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Polymerase acidic protein	polymer	622	71623.4	1	UniProt (P27194) Pfam (PF00603) In PDB	Thogoto virus (isolate SiAr 126) (Tho)	PA,RNA-directed RNA polymerase subunit P3
2	B	RNA-directed RNA polymerase catalytic subunit	polymer	710	81432.7	1	UniProt (O41353) Pfam (PF00602) In PDB	Thogoto virus (isolate SiAr 126) (Tho)	RNA-directed RNA polymerase subunit P2
3	C	Polymerase basic protein 2	polymer	827	94418.6	1	UniProt (Q9YNA4) Pfam (PF20950) Pfam (PF21490) Pfam (PF21442) In PDB	Thogoto virus (isolate SiAr 126) (Tho)	PB2,RNA-directed RNA polymerase subunit P3
4	D	RNA (5'-R(*AP*GP*AP*GP*AP*AP*AP*UP*CP*AP*AP*GP*GP*CP*AP*GP*UP*U)-3')	polymer	18	5843.6	1		Thogoto virus (isolate SiAr 126)
5	E	RNA (5'-R(*GP*AP*CP*UP*GP*CP*CP*UP*GP*UP*UP*UP*UP*UP*GP*CP*U)-3')	polymer	17	5335.1	1		Thogoto virus (isolate SiAr 126)
6	B	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER	non-polymer		521.2	1	Chemie (G2P)

Sequence modifications

A: 1 - 622 (UniProt: P27194)

PDB	External Database	Details
Glu 471	Gly 471	conflict

B: 1 - 710 (UniProt: O41353)

PDB	External Database	Details
Leu 7	Arg 7	conflict
Trp 230	Cys 230	conflict

C: 1 - 769 (UniProt: Q9YNA4)

PDB	External Database	Details
Leu 770	-	expression tag
Glu 771	-	expression tag
Val 772	-	expression tag
Leu 773	-	expression tag
Phe 774	-	expression tag
Gln 775	-	expression tag
Gly 776	-	expression tag
Pro 777	-	expression tag
Gly 778	-	expression tag
His 779	-	expression tag
His 780	-	expression tag
His 781	-	expression tag
His 782	-	expression tag
His 783	-	expression tag
His 784	-	expression tag
His 785	-	expression tag
His 786	-	expression tag
Ser 787	-	expression tag
Ala 788	-	expression tag
Asp 789	-	expression tag
Tyr 790	-	expression tag
Lys 791	-	expression tag
Asp 792	-	expression tag
Asp 793	-	expression tag
Asp 794	-	expression tag
Asp 795	-	expression tag
Lys 796	-	expression tag
Gly 797	-	expression tag
Gly 798	-	expression tag
Trp 799	-	expression tag
Ser 800	-	expression tag
His 801	-	expression tag
Pro 802	-	expression tag
Gln 803	-	expression tag
Phe 804	-	expression tag
Glu 805	-	expression tag
Lys 806	-	expression tag
Gly 807	-	expression tag
Gly 808	-	expression tag
Gly 809	-	expression tag
Ser 810	-	expression tag
Gly 811	-	expression tag
Gly 812	-	expression tag
Gly 813	-	expression tag
Gly 814	-	expression tag
Ser 815	-	expression tag
Gly 816	-	expression tag
Gly 817	-	expression tag
Ser 818	-	expression tag
Ala 819	-	expression tag
Trp 820	-	expression tag
Ser 821	-	expression tag
His 822	-	expression tag
Pro 823	-	expression tag
Gln 824	-	expression tag
Phe 825	-	expression tag
Glu 826	-	expression tag
Lys 827	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	258653.4
Non-Polymers*	Number of molecules	1
	Total formula weight	521.2
All*	Total formula weight	259174.6

*Water molecules are not included.