8Z8A
Cryo-EM structure of the Lactate-bound human HCAR1-Gi1 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Hydroxycarboxylic acid receptor 1 | polymer | 346 | 39338.3 | 1 | UniProt (Q9BXC0) Pfam (PF00001) | Homo sapiens (human) | G-protein coupled receptor 104,G-protein coupled receptor 81 |
| 2 | B (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 70 | 7729.9 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | E (S) | single Fab chain (scFv16) | polymer | 250 | 26610.6 | 1 | synthetic construct | ||
| 6 | F (R) | (2S)-2-HYDROXYPROPANOIC ACID | non-polymer | 90.1 | 1 | Chemie (2OP) |
Sequence modifications
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | conflict |
| Ala 203 | Gly 203 | conflict |
| Ala 245 | Glu 245 | conflict |
| Ser 326 | Ala 326 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 152008.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 90.1 | |
| All* | Total formula weight | 152098.4 |
