8Z7P

Cryo-EM structure of SARS-CoV-2 S trimer in the early fusion intermediate conformation (E-FIC)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (D, E, F)	Angiotensin-converting enzyme 2	polymer	631	72989.0	3	UniProt (Q9BYF1) Pfam (PF01401)	Homo sapiens (human)	Angiotensin-converting enzyme homolog,ACEH,Angiotensin-converting enzyme-related carboxypeptidase,ACE-related carboxypeptidase,Metalloprotease MPROT15
2	B, D, F (A, B, C)	Spike glycoprotein	polymer	1288	142535.6	3	UniProt (P0DTC2) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601)	Severe acute respiratory syndrome coronavirus 2	S glycoprotein,E2,Peplomer protein
3	AA, BA, CA, G, H... (a, b, c, G, H...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	21	In PDB GlyTouCan (G42666HT)
4	DA, N, V (d, N, V)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
5	AB, BB, CB, DB, EA... (B, D, F, A, C...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	42	Chemie (NAG)

Sequence modifications

D, E, F: 1 - 615 (UniProt: Q9BYF1)

PDB	External Database	Details
Leu 616	-	expression tag
Glu 617	-	expression tag
Val 618	-	expression tag
Leu 619	-	expression tag
Phe 620	-	expression tag
Gln 621	-	expression tag
Gly 622	-	expression tag
Pro 623	-	expression tag
His 624	-	expression tag
His 625	-	expression tag
His 626	-	expression tag
His 627	-	expression tag
His 628	-	expression tag
His 629	-	expression tag
His 630	-	expression tag
His 631	-	expression tag

A, B, C: 1 - 1208 (UniProt: P0DTC2)

PDB	External Database	Details
Gly 614	Asp 614	variant
Gly 1209	-	expression tag
Ser 1210	-	expression tag
Gly 1211	-	expression tag
Tyr 1212	-	expression tag
Ile 1213	-	expression tag
Pro 1214	-	expression tag
Glu 1215	-	expression tag
Ala 1216	-	expression tag
Pro 1217	-	expression tag
Arg 1218	-	expression tag
Asp 1219	-	expression tag
Gly 1220	-	expression tag
Gln 1221	-	expression tag
Ala 1222	-	expression tag
Tyr 1223	-	expression tag
Val 1224	-	expression tag
Arg 1225	-	expression tag
Lys 1226	-	expression tag
Asp 1227	-	expression tag
Gly 1228	-	expression tag
Glu 1229	-	expression tag
Trp 1230	-	expression tag
Val 1231	-	expression tag
Phe 1232	-	expression tag
Leu 1233	-	expression tag
Ser 1234	-	expression tag
Thr 1235	-	expression tag
Phe 1236	-	expression tag
Leu 1237	-	expression tag
Ser 1238	-	expression tag
Gly 1239	-	expression tag
Leu 1240	-	expression tag
Glu 1241	-	expression tag
Val 1242	-	expression tag
Leu 1243	-	expression tag
Phe 1244	-	expression tag
Gln 1245	-	expression tag
Gly 1246	-	expression tag
Pro 1247	-	expression tag
Gly 1248	-	expression tag
Gly 1249	-	expression tag
Trp 1250	-	expression tag
Ser 1251	-	expression tag
His 1252	-	expression tag
Pro 1253	-	expression tag
Gln 1254	-	expression tag
Phe 1255	-	expression tag
Glu 1256	-	expression tag
Lys 1257	-	expression tag
Gly 1258	-	expression tag
Gly 1259	-	expression tag
Gly 1260	-	expression tag
Ser 1261	-	expression tag
Gly 1262	-	expression tag
Gly 1263	-	expression tag
Gly 1264	-	expression tag
Ser 1265	-	expression tag
Gly 1266	-	expression tag
Gly 1267	-	expression tag
Ser 1268	-	expression tag
Ala 1269	-	expression tag
Trp 1270	-	expression tag
Ser 1271	-	expression tag
His 1272	-	expression tag
Pro 1273	-	expression tag
Gln 1274	-	expression tag
Phe 1275	-	expression tag
Glu 1276	-	expression tag
Lys 1277	-	expression tag
Gly 1278	-	expression tag
Gly 1279	-	expression tag
Ser 1280	-	expression tag
His 1281	-	expression tag
His 1282	-	expression tag
His 1283	-	expression tag
His 1284	-	expression tag
His 1285	-	expression tag
His 1286	-	expression tag
His 1287	-	expression tag
His 1288	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	646574.1
Branched	Number of molecules	24
	Total formula weight	10672.0
Non-Polymers*	Number of molecules	42
	Total formula weight	9290.7
All*	Total formula weight	666536.8

*Water molecules are not included.