8Z7N

Structure of HIV-1 CH119 SOSIP.664 trimer in complex with CD4 molecules

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G (A, D, G)	Envelope glycoprotein gp160	polymer	518	58258.1	3	UniProt (A1EAH4)	Human immunodeficiency virus 1	Env polyprotein
2	B, E, H (B, E, H)	Envelope glycoprotein gp160	polymer	164	18285.7	3	UniProt (A1EAH4)	Human immunodeficiency virus 1	Env polyprotein
3	C, F, I (C, F, I)	T-cell surface glycoprotein CD4	polymer	401	44675.3	3	UniProt (P01730) Pfam (PF00047) Pfam (PF05790) Pfam (PF09191)	Homo sapiens (human)	T-cell surface antigen T4/Leu-3
4	AA, J, K, L, M... (H, A, B, D, E...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	18	Chemie (NAG) PubChem (24139)

Sequence modifications

A, D, G: 36 - 514 (UniProt: A1EAH4)

PDB	External Database	Details
Met 1	-	initiating methionine
Asp 2	-	expression tag
Ala 3	-	expression tag
Met 4	-	expression tag
Lys 5	-	expression tag
Arg 6	-	expression tag
Gly 7	-	expression tag
Leu 8	-	expression tag
Cys 9	-	expression tag
Cys 10	-	expression tag
Val 11	-	expression tag
Leu 12	-	expression tag
Leu 13	-	expression tag
Leu 14	-	expression tag
Cys 15	-	expression tag
Gly 16	-	expression tag
Ala 17	-	expression tag
Val 18	-	expression tag
Phe 19	-	expression tag
Val 20	-	expression tag
Ser 21	-	expression tag
Pro 22	-	expression tag
Ser 23	-	expression tag
Gln 24	-	expression tag
Glu 25	-	expression tag
Ile 26	-	expression tag
His 27	-	expression tag
Ala 28	-	expression tag
Arg 29	-	expression tag
Phe 30	-	expression tag
Arg 31	-	expression tag
Arg 32	-	expression tag
Gly 33	-	expression tag
Ala 34	-	expression tag
Arg 35	-	expression tag
Cys 507	Ala 499	engineered mutation
Arg 515	-	expression tag
Arg 516	-	expression tag
Arg 517	-	expression tag
Arg 518	-	expression tag
Arg 519	-	expression tag

B, E, H: 519 - 671 (UniProt: A1EAH4)

PDB	External Database	Details
Pro 566	Ile 557	engineered mutation
Cys 612	Thr 603	engineered mutation
Gly 672	-	expression tag
Gly 673	-	expression tag
Gly 674	-	expression tag
Gly 675	-	expression tag
Gly 676	-	expression tag
His 677	-	expression tag
His 678	-	expression tag
His 679	-	expression tag
His 680	-	expression tag
His 681	-	expression tag
His 682	-	expression tag

C, F, I: -22 - 369 (UniProt: P01730)

PDB	External Database	Details
Gly 370	-	expression tag
Ser 371	-	expression tag
Gly 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	363657.4
Non-Polymers*	Number of molecules	18
	Total formula weight	3981.7
All*	Total formula weight	367639.2

*Water molecules are not included.