8Z76

The structure of thiocyanate dehydrogenase from Pelomicrobium methylotrophicum (pmTcDH), activated by crystals soaking with 1 mM CuCl2 during 6 months

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Twin-arginine translocation signal domain-containing protein	polymer	489	54093.3	3	UniProt (A0A5C7ETD9)	Pelomicrobium methylotrophicum
2	D, E, K, L, P (A, B, C)	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
3	F, G, H, I, M... (A, B, C)	COPPER (II) ION	non-polymer		63.5	10	Chemie (CU)
4	J (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	T, U, V (A, B, C)	water	water		18.0	801	Chemie (HOH)

Sequence modifications

A, B, C: 46 - 513 (UniProt: A0A5C7ETD9)

PDB	External Database	Details
Met 25	-	initiating methionine
Gly 26	-	expression tag
Ser 27	-	expression tag
Asp 28	-	expression tag
Lys 29	-	expression tag
Ile 30	-	expression tag
His 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
Glu 37	-	expression tag
Asn 38	-	expression tag
Leu 39	-	expression tag
Tyr 40	-	expression tag
Phe 41	-	expression tag
Gln 42	-	expression tag
Gly 43	-	expression tag
His 44	-	expression tag
Met 45	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	162279.9
Non-Polymers*	Number of molecules	16
	Total formula weight	968.8
All*	Total formula weight	163248.7

*Water molecules are not included.