8Z2H

Substrate analog a010 bound form of PET-degrading cutinase mutant Cut190**SS_S176A

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Alpha/beta hydrolase family protein	polymer	260	28533.1	2	UniProt (W0TJ64) Pfam (PF03403)	Saccharomonospora viridis	cutinase
2	C, E (A, B)	4-[2-hydroxyethyloxy(oxidanyl)phosphoryl]benzoic acid	non-polymer		246.2	2	Chemie (A1L0N)
3	D, F (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	G, H (A, B)	water	water		18.0	199	Chemie (HOH)

Sequence modifications

A, B: 47 - 304 (UniProt: W0TJ64)

PDB	External Database	Details
Gly 45	-	expression tag
Pro 46	-	expression tag
His 123	Gln 123	engineered mutation
Ala 138	Gln 138	engineered mutation
Ala 176	Ser 176	engineered mutation
His 202	Asn 202	engineered mutation
Pro 226	Ser 226	engineered mutation
Ser 228	Arg 228	engineered mutation
Cys 250	Asp 250	engineered mutation
Cys 296	Glu 296	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57066.2
Non-Polymers*	Number of molecules	4
	Total formula weight	572.5
All*	Total formula weight	57638.7

*Water molecules are not included.