8Z2G
MHET bound form of PET-degrading cutinase mutant Cut190*SS_S176A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Alpha/beta hydrolase family protein | polymer | 263 | 28889.5 | 2 | UniProt (W0TJ64) Pfam (PF03403) | Saccharomonospora viridis | Cutinase |
| 2 | C (A) | AMMONIUM ION | non-polymer | 18.0 | 1 | Chemie (NH4) | |||
| 3 | D (A) | 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 209.2 | 1 | Chemie (BTB) | |||
| 4 | E, G (A, B) | 4-(2-hydroxyethyloxycarbonyl)benzoic acid | non-polymer | 210.2 | 2 | Chemie (C9C) | |||
| 5 | F (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | H, I (A, B) | water | water | 18.0 | 258 | Chemie (HOH) |
Sequence modifications
A, B: 47 - 304 (UniProt: W0TJ64)
| PDB | External Database | Details |
|---|---|---|
| Gly 45 | - | expression tag |
| Pro 46 | - | expression tag |
| His 123 | Gln 123 | engineered mutation |
| Ala 138 | Gln 138 | engineered mutation |
| Ala 176 | Ser 176 | engineered mutation |
| His 202 | Asn 202 | engineered mutation |
| Pro 226 | Ser 226 | engineered mutation |
| Ser 228 | Arg 228 | engineered mutation |
| Cys 250 | Asp 250 | engineered mutation |
| Cys 296 | Glu 296 | engineered mutation |
| Lys 305 | - | expression tag |
| Leu 306 | - | expression tag |
| Asn 307 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 57779.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 739.7 | |
| All* | Total formula weight | 58518.8 |
