8YYQ

Structure of the HitB F328L mutant

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Putative ATP-dependent b-aminoacyl-ACP synthetase	polymer	549	59641.6	1	UniProt (A0A0F7R6G7) Pfam (PF00501) Pfam (PF13193) In PDB	Embleya scabrispora
2	A	[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl ~{N}-[(3~{S})-3-azanyl-3-(3-cyanophenyl)propanoyl]sulfamate	non-polymer		518.5	1	Chemie (A1L0G)
3		water	water		18.0	223	Chemie (HOH)

Sequence modifications

A: 1 - 533 (UniProt: A0A0F7R6G7)

PDB	External Database	Details
Met -15	-	initiating methionine
Asn -14	-	expression tag
His -13	-	expression tag
Lys -12	-	expression tag
Val -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Ile -4	-	expression tag
Glu -3	-	expression tag
Gly -2	-	expression tag
Arg -1	-	expression tag
His 0	-	expression tag
Leu 328	Phe 328	engineered mutation
Asn 471	Leu 471	conflict

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	59641.6
Non-Polymers*	Number of molecules	1
	Total formula weight	518.5
All*	Total formula weight	60160.1

*Water molecules are not included.