8YYJ

RNase J2 mutant H80A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Ribonuclease J 2	polymer	571	64312.3	4	UniProt (Q5HPR6) Pfam (PF00753) Pfam (PF22505) Pfam (PF07521) Pfam (PF17770) UniProt (by SIFTS) (Q2FZ19)	Staphylococcus aureus	RNase J2
2	E, F, G, H (A, B, C, D)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
3	I, J, K, L (A, B, C, D)	water	water		18.0	8	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 557 (UniProt: Q5HPR6)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Ala 80	His 80	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	257249.0
Non-Polymers*	Number of molecules	4
	Total formula weight	219.8
All*	Total formula weight	257468.8

*Water molecules are not included.