8YYH

RNase J2 mutant H144A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ribonuclease J 2	polymer	571	64312.3	1	UniProt (Q5HPR6) Pfam (PF00753) Pfam (PF22505) Pfam (PF07521) Pfam (PF17770) UniProt (by SIFTS) (Q2FZ19)	Staphylococcus aureus	RNase J2
2	B (A)	water	water		18.0	273	Chemie (HOH)

Sequence modifications

A: 1 - 557 (UniProt: Q5HPR6)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Ala 144	His 144	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	64312.3
All*	Total formula weight	64312.3

*Water molecules are not included.