8YWF

Cryo-EM structure of GLP1 complex bound with Retatrutide

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	361	41879.5	1	UniProt (P63096) UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein,Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	397	43299.3	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	NB35	polymer	132	14364.0	1		Homo sapiens
5	E (P)	Retatrutide	polymer	33	3629.0	1		Homo sapiens
6	F (R)	Glucagon-like peptide 1 receptor	polymer	440	50860.8	1	UniProt (P43220) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (human)	GLP-1 receptor,GLP-1-R,GLP-1R
7	G (R)	20-[[(2~{S})-1-oxidanyl-1,5-bis(oxidanylidene)-5-[2-[2-(2-oxidanylideneethoxy)ethoxy]ethylamino]pentan-2-yl]amino]-20-oxidanylidene-icosanoic acid	non-polymer		616.8	1	Chemie (A1D7Q) PubChem (139452618)

Sequence modifications

A: 1 - 19 (UniProt: P63096)
A: 20 - 56 (UniProt: P63092)

PDB	External Database	Details
Asp 42	Gly 49	conflict
Asn 43	Glu 50	conflict
Tyr 56	Leu 63	conflict

A: 56 - 98 (UniProt: P63096)
A: 99 - 147 (UniProt: P63092)

PDB	External Database	Details
Ala 120	Gly 226	conflict
Asp 143	Ala 249	conflict
Asp 146	Ser 252	conflict

A: 148 - 278 (UniProt: P63092)

PDB	External Database	Details
Asp 156	Leu 272	conflict
Ser 250	Ala 366	conflict
Ala 256	Ile 372	conflict
Ile 259	Val 375	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
Ser -26	-	expression tag
Ser -25	-	expression tag
Gly -24	-	expression tag
Leu -23	-	expression tag
Val -22	-	expression tag
Pro -21	-	expression tag
Arg -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Met -16	-	expression tag
Ala -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	161893.7
Non-Polymers*	Number of molecules	1
	Total formula weight	616.8
All*	Total formula weight	162510.5

*Water molecules are not included.