8YW8

Cryo-EM structure of human mitochondrial pyruvate carrier in complex with the inhibitor UK5099

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Mitochondrial pyruvate carrier 2	polymer	151	17161.7	1	UniProt (O95563) Pfam (PF03650)	Homo sapiens (human)	Brain protein 44
2	B (C)	MPC specific nanobody 1	polymer	138	15255.8	1		Escherichia coli BL21(DE3)
3	C (A)	Mitochondrial pyruvate carrier 1	polymer	120	13592.8	1	UniProt (Q9Y5U8) Pfam (PF03650)	Homo sapiens (human)	Brain protein 44-like protein
4	D (B)	CARDIOLIPIN	non-polymer		1464.0	1	Chemie (CDL)
5	E (A)	(E)-2-cyano-3-(1-phenylindol-3-yl)prop-2-enoic acid	non-polymer		288.3	1	Chemie (I2R)

Sequence modifications

B: 1 - 127 (UniProt: O95563)

PDB	External Database	Details
Gly 128	-	expression tag
Ser 129	-	expression tag
Asp 130	-	expression tag
Tyr 131	-	expression tag
Lys 132	-	expression tag
Asp 133	-	expression tag
His 134	-	expression tag
Asp 135	-	expression tag
Gly 136	-	expression tag
Asp 137	-	expression tag
Tyr 138	-	expression tag
Lys 139	-	expression tag
Asp 140	-	expression tag
His 141	-	expression tag
Asp 142	-	expression tag
Ile 143	-	expression tag
Asp 144	-	expression tag
Tyr 145	-	expression tag
Lys 146	-	expression tag
Asp 147	-	expression tag
Asp 148	-	expression tag
Asp 149	-	expression tag
Asp 150	-	expression tag
Lys 151	-	expression tag

A: 1 - 109 (UniProt: Q9Y5U8)

PDB	External Database	Details
Gly 110	-	expression tag
Ser 111	-	expression tag
Tyr 112	-	expression tag
Pro 113	-	expression tag
Tyr 114	-	expression tag
Asp 115	-	expression tag
Val 116	-	expression tag
Pro 117	-	expression tag
Asp 118	-	expression tag
Tyr 119	-	expression tag
Ala 120	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	46010.3
Non-Polymers*	Number of molecules	2
	Total formula weight	1752.3
All*	Total formula weight	47762.7

*Water molecules are not included.