8YW5
Cryo-EM structure of the retatrutide-bound human GCGR-Gs complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | P | Retatrutide | polymer | 30 | 3357.8 | 1 | Homo sapiens | ||
2 | R | Glucagon receptor | polymer | 406 | 46741.4 | 1 | UniProt (P47871) Pfam (PF02793) Pfam (PF00002) In PDB | Homo sapiens (human) | GL-R |
3 | A | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45683.4 | 1 | UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
4 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
5 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
6 | N | Nanobody-35 | polymer | 140 | 15343.0 | 1 | synthetic construct |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
PDB | External Database | Details |
---|---|---|
Asn 54 | Ser 54 | engineered mutation |
Ala 226 | Gly 226 | engineered mutation |
Ala 268 | Glu 268 | engineered mutation |
Lys 271 | Asn 271 | engineered mutation |
Asp 274 | Lys 274 | engineered mutation |
Lys 280 | Arg 280 | engineered mutation |
Asp 284 | Thr 284 | engineered mutation |
Thr 285 | Ile 285 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -4 | - | initiating methionine |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Leu -1 | - | expression tag |
Leu 0 | - | expression tag |
Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 156902.2 | |
All* | Total formula weight | 156902.2 |