8YVS

Crystal structure of GH65 alpha-1,2-glucosidase from Flavobacterium johnsoniae in complex with castanospermine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65	polymer	678	76489.2	3	UniProt (A5FBJ5) Pfam (PF03636) Pfam (PF03632)	Flavobacterium johnsoniae UW101	alpha-1,2-glucosidase
2	D, E, J, K, M... (A, B, C)	CASTANOSPERMINE	non-polymer		189.2	6	Chemie (CTS)
3	F, G, H, I, L... (A, B, C)	1,2-ETHANEDIOL	non-polymer		62.1	8	Chemie (EDO)
4	R, S, T (A, B, C)	water	water		18.0	1204	Chemie (HOH)

Sequence modifications

A, B, C: 24 - 681 (UniProt: A5FBJ5)

PDB	External Database	Details
Met 4	-	initiating methionine
Gly 5	-	expression tag
Ser 6	-	expression tag
Ser 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
Ser 14	-	expression tag
Ser 15	-	expression tag
Gly 16	-	expression tag
Leu 17	-	expression tag
Val 18	-	expression tag
Pro 19	-	expression tag
Arg 20	-	expression tag
Gly 21	-	expression tag
Ser 22	-	expression tag
His 23	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	229467.7
Non-Polymers*	Number of molecules	14
	Total formula weight	1631.8
All*	Total formula weight	231099.5

*Water molecules are not included.