8YVR

Crystal structure of GH65 alpha-1,2-glucosidase from Flavobacterium johnsoniae in complex with 1-deoxynojirimycin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65	polymer	678	76489.2	3	UniProt (A5FBJ5)	Flavobacterium johnsoniae UW101	alpha-1,2-glucosidase
2	D, F, G (A, B, C)	1-DEOXYNOJIRIMYCIN	non-polymer		163.2	3	Chemie (NOJ)
3	E, H (A, C)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
4	I, J, K (A, B, C)	water	water		18.0	886	Chemie (HOH)

Sequence modifications

A, B, C: 24 - 681 (UniProt: A5FBJ5)

PDB	External Database	Details
Met 4	-	initiating methionine
Gly 5	-	expression tag
Ser 6	-	expression tag
Ser 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
Ser 14	-	expression tag
Ser 15	-	expression tag
Gly 16	-	expression tag
Leu 17	-	expression tag
Val 18	-	expression tag
Pro 19	-	expression tag
Arg 20	-	expression tag
Gly 21	-	expression tag
Ser 22	-	expression tag
His 23	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	229467.7
Non-Polymers*	Number of molecules	5
	Total formula weight	613.7
All*	Total formula weight	230081.3

*Water molecules are not included.