8YU5
The structure of non-activated thiocyanate dehydrogenase mutant with the H447Q substitution from Pelomicrobium methylotrophicum (pmTcDH H447Q)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Twin-arginine translocation signal domain-containing protein | polymer | 489 | 54083.3 | 2 | UniProt (A0A5C7ETD9) | Pelomicrobium methylotrophicum | |
| 2 | C (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 3 | D, E, F, J, K... (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 6 | Chemie (EDO) | |||
| 4 | G, H, I, M, N (A, B) | COPPER (II) ION | non-polymer | 63.5 | 5 | Chemie (CU) | |||
| 5 | O (B) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | P, Q (A, B) | water | water | 18.0 | 1044 | Chemie (HOH) |
Sequence modifications
A, B: 46 - 513 (UniProt: A0A5C7ETD9)
| PDB | External Database | Details |
|---|---|---|
| Met 25 | - | initiating methionine |
| Gly 26 | - | expression tag |
| Ser 27 | - | expression tag |
| Asp 28 | - | expression tag |
| Lys 29 | - | expression tag |
| Ile 30 | - | expression tag |
| His 31 | - | expression tag |
| His 32 | - | expression tag |
| His 33 | - | expression tag |
| His 34 | - | expression tag |
| His 35 | - | expression tag |
| His 36 | - | expression tag |
| Glu 37 | - | expression tag |
| Asn 38 | - | expression tag |
| Leu 39 | - | expression tag |
| Tyr 40 | - | expression tag |
| Phe 41 | - | expression tag |
| Gln 42 | - | expression tag |
| Gly 43 | - | expression tag |
| His 44 | - | expression tag |
| Met 45 | - | expression tag |
| Gln 447 | His 447 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 108166.6 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 831.7 | |
| All* | Total formula weight | 108998.3 |
