8YTY

The M12+P185V variant of Kubu-PETase from Kutzneria buriramensis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Dienelactone hydrolase	polymer	260	27042.3	1	UniProt (A0A3E0H050)	Kutzneria buriramensis	Recombinant Kubu-P-M12+P185V variant
2	B (A)	water	water		18.0	367	Chemie (HOH)

Sequence modifications

A: 38 - 288 (UniProt: A0A3E0H050)

PDB	External Database	Details
Met 37	-	initiating methionine
Arg 95	Thr 95	variant
Asn 119	Thr 119	variant
Ser 127	Gln 127	variant
Gln 131	Glu 131	variant
Cys 173	Ala 173	variant
Ile 184	Val 184	variant
Val 185	Pro 185	variant
His 190	Asp 190	variant
Cys 197	Lys 197	variant
Cys 236	Ala 236	variant
Ser 239	Asp 239	variant
Ser 279	Ala 279	variant
Cys 281	Ser 281	variant
Leu 289	-	expression tag
Glu 290	-	expression tag
His 291	-	expression tag
His 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27042.3
All*	Total formula weight	27042.3

*Water molecules are not included.