8YRS

Crystal structure of human RECQ1 helicase containing a flexible linker in complex with tailed duplex DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ATP-dependent DNA helicase Q1	polymer	599	67408.0	2	UniProt (P46063) Pfam (PF00270) Pfam (PF00271) Pfam (PF16124)	Homo sapiens (human)	DNA helicase,RecQ-like type 1,RecQ1,DNA-dependent ATPase Q1,RecQ protein-like 1
2	C, E (C, P)	DNA (5'-D(*AP*GP*CP*GP*TP*CP*GP*AP*GP*AP*TP*CP*C)-3')	polymer	13	3976.6	2		Homo sapiens
3	D, F (D, Q)	DNA (27-MER)	polymer	27	8210.3	2		Homo sapiens
4	G, H (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, B: 49 - 480 (UniProt: P46063)

PDB	External Database	Details
Met 28	-	initiating methionine
Gly 29	-	expression tag
Ser 30	-	expression tag
Ser 31	-	expression tag
His 32	-	expression tag
His 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
His 37	-	expression tag
Ser 38	-	expression tag
Ser 39	-	expression tag
Gly 40	-	expression tag
Leu 41	-	expression tag
Val 42	-	expression tag
Pro 43	-	expression tag
Arg 44	-	expression tag
Gly 45	-	expression tag
Ser 46	-	expression tag
His 47	-	expression tag
Met 48	-	expression tag

A, B: 491 - 626 (UniProt: P46063)

PDB	External Database	Details
Gly 481	-	linker
Gly 482	-	linker
Gly 483	-	linker
Gly 484	-	linker
Ser 485	-	linker
Gly 486	-	linker
Gly 487	-	linker
Gly 488	-	linker
Gly 489	-	linker
Ser 490	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	159189.8
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	159320.6

*Water molecules are not included.