8YP4

Structure of MAP2K1 complexed with 5Z7-oxozeaenol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Dual specificity mitogen-activated protein kinase kinase 1	polymer	365	40836.0	2	UniProt (Q02750) Pfam (PF00069)	Homo sapiens (human)	MAP kinase kinase 1,MAPKK 1,MKK1,ERK activator kinase 1,MAPK/ERK kinase 1,MEK 1
2	C, E (A, B)	(4~{S},9~{S},10~{S},12~{E})-16-methoxy-4-methyl-9,10,18-tris(oxidanyl)-3-oxabicyclo[12.4.0]octadeca-1(18),12,14,16-tetraene-2,8-dione	non-polymer		364.4	2	Chemie (WNT)
3	D (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	F, G (A, B)	water	water		18.0	137	Chemie (HOH)

Sequence modifications

A, B: 34 - 386 (UniProt: Q02750)

PDB	External Database	Details
Met 33	-	initiating methionine
Ala 286	Thr 286	engineered mutation
Ala 292	Thr 292	engineered mutation
Ala 298	Ser 298	engineered mutation
Ala 387	-	expression tag
Ala 388	-	expression tag
Ala 389	-	expression tag
Leu 390	-	expression tag
Glu 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	81672.0
Non-Polymers*	Number of molecules	3
	Total formula weight	820.9
All*	Total formula weight	82492.9

*Water molecules are not included.