8YO7

structure of phage T6 topoisomerase II central domain bound with DNA and m-AMSA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA topoisomerase medium subunit	polymer	452	51952.0	2	UniProt (P07065) Pfam (PF00521)	Escherichia phage T4	DNA topoisomerase 51-kDa subunit,Protein Gp52
2	C, D (C, D)	DNA topoisomerase (ATP-hydrolyzing)	polymer	611	69237.3	2	UniProt (A0A346FJ89) Pfam (PF02518) Pfam (PF00204)	Enterobacteria phage T6	phage T6 topoisomerase II gp39 subunit
3	E, H (E, Y)	DNA (5'-D(P*AP*TP*AP*TP*AP*CP*AP*CP*AP*TP*AP*T)-3')	polymer	52	16058.4	2		DNA molecule
4	F, G (F, X)	DNA (5'-D(P*AP*TP*AP*TP*AP*TP*AP*CP*AP*CP*A)-3')	polymer	52	15965.4	2		DNA molecule
5	I, J (C, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	K, L (E, F)	N-[4-(acridin-9-ylamino)-3-methoxyphenyl]methanesulfonamide	non-polymer		393.5	2	Chemie (ASW)
7	M, N (X, Y)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A, B: 1 - 442 (UniProt: P07065)

PDB	External Database	Details
His 443	-	expression tag
His 444	-	expression tag
His 445	-	expression tag
His 446	-	expression tag
His 447	-	expression tag
His 448	-	expression tag
His 449	-	expression tag
His 450	-	expression tag
His 451	-	expression tag
His 452	-	expression tag

C, D: 1 - 605 (UniProt: A0A346FJ89)

PDB	External Database	Details
His 606	-	expression tag
His 607	-	expression tag
His 608	-	expression tag
His 609	-	expression tag
His 610	-	expression tag
His 611	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	306426.0
Non-Polymers*	Number of molecules	4
	Total formula weight	835.5
All*	Total formula weight	307261.6

*Water molecules are not included.