8YNW

S102A mutant of poly(3-hydroxybutyrate) depolymerase PhaZ from Bacillus thuringiensis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Poly(3-hydroxybutyrate) depolymerase	polymer	311	35545.3	4	UniProt (A0A9X6EQW5) Pfam (PF00561) UniProt (by SIFTS) (A0A9W3JUC0)	Bacillus thuringiensis	Alpha/beta hydrolase
2	E, F (B, D)	3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL	non-polymer		326.4	2	Chemie (P33)
3	G (D)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
4	H, I, J, K (A, B, C, D)	water	water		18.0	959	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 300 (UniProt: A0A9X6EQW5)

PDB	External Database	Details
Met -10	-	initiating methionine
Arg -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Ala 102	Ser 102	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	142181.4
Non-Polymers*	Number of molecules	3
	Total formula weight	774.9
All*	Total formula weight	142956.3

*Water molecules are not included.