8YN7

Cryo-EM structure of histamine H3 receptor in complex with immethridine and miniGo

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Engineered guanine nucleotide-binding protein G(o) subunit alpha,Guanine nucleotide-binding protein G(o) subunit alpha	polymer	226	25286.9	1	UniProt (P09471) Pfam (PF00503)	synthetic construct
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	376	40845.6	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (E)	Antibody fragment scFv16	polymer	255	27314.4	1		synthetic construct
5	E (R)	Histamine H3 receptor	polymer	659	72568.7	1	UniProt (Q9Y5N1) Pfam (PF00001)	Homo sapiens (human)
6	F (R)	4-(1H-imidazol-4-ylmethyl)pyridine	non-polymer		159.2	1	Chemie (A1LY2)
7	G, H, I, J, K (R)	CHOLESTEROL	non-polymer		386.7	5	Chemie (CLR)

Sequence modifications

A: 3 - 181 (PDB: 8YN7)
A: 182 - 354 (UniProt: P09471)

PDB	External Database	Details
Asp 227	Ala 227	engineered mutation
Asp 230	Gly 230	engineered mutation
-	Asp 232	deletion
-	Gln 233	deletion
-	Val 234	deletion
-	Leu 235	deletion
-	His 236	deletion
-	Glu 237	deletion
-	Asp 238	deletion
-	Glu 239	deletion
-	Thr 240	deletion
-	Thr 241	deletion
Ala 332	Ile 332	engineered mutation
Ile 335	Val 335	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: 1 - 445 (UniProt: Q9Y5N1)

PDB	External Database	Details
Asp -52	-	expression tag
Tyr -51	-	expression tag
Lys -50	-	expression tag
Asp -49	-	expression tag
Asp -48	-	expression tag
Asp -47	-	expression tag
Asp -46	-	expression tag
His -45	-	expression tag
His -44	-	expression tag
His -43	-	expression tag
His -42	-	expression tag
His -41	-	expression tag
His -40	-	expression tag
His -39	-	expression tag
His -38	-	expression tag
Gly -37	-	expression tag
Gln -36	-	expression tag
Pro -35	-	expression tag
Gly -34	-	expression tag
Asn -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Ala -30	-	expression tag
Phe -29	-	expression tag
Leu -28	-	expression tag
Leu -27	-	expression tag
Ala -26	-	expression tag
Pro -25	-	expression tag
Asn -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
His -21	-	expression tag
Ala -20	-	expression tag
Pro -19	-	expression tag
Asp -18	-	expression tag
His -17	-	expression tag
Asn -16	-	expression tag
Val -15	-	expression tag
Thr -14	-	expression tag
Gln -13	-	expression tag
Gln -12	-	expression tag
Arg -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Val -1	-	expression tag
Asp 0	-	expression tag

R: 446 - 606 (PDB: 8YN7)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	173876.7
Non-Polymers*	Number of molecules	6
	Total formula weight	2092.5
All*	Total formula weight	175969.2

*Water molecules are not included.