8YN3

Cryo-EM structure of histamine H2 receptor in complex with histamine and miniGs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Engineered guanine nucleotide,binding protein G(s) subunit alpha	polymer	246	29048.9	1		synthetic construct
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	376	40845.6	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody Nb35	polymer	138	15140.7	1		synthetic construct
5	E (R)	Histamine H2 receptor	polymer	568	62465.4	1	UniProt (P25021) Pfam (PF00001)	Homo sapiens (human)	H2R,HH2R,Gastric receptor I
6	F (R)	HISTAMINE	non-polymer		111.1	1	Chemie (HSM)
7	G (R)	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)

Sequence modifications

A: 1 - 65 (PDB: 8YN3)
A: 66 - 394 (PDB: 8YN3)
B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: 1 - 308 (UniProt: P25021)

PDB	External Database	Details
Asp -84	-	expression tag
Tyr -83	-	expression tag
Lys -82	-	expression tag
Asp -81	-	expression tag
Asp -80	-	expression tag
Asp -79	-	expression tag
Asp -78	-	expression tag
His -77	-	expression tag
His -76	-	expression tag
His -75	-	expression tag
His -74	-	expression tag
His -73	-	expression tag
His -72	-	expression tag
His -71	-	expression tag
His -70	-	expression tag
Gly -69	-	expression tag
Gln -68	-	expression tag
Pro -67	-	expression tag
Gly -66	-	expression tag
Asn -65	-	expression tag
Gly -64	-	expression tag
Ser -63	-	expression tag
Ala -62	-	expression tag
Phe -61	-	expression tag
Leu -60	-	expression tag
Leu -59	-	expression tag
Ala -58	-	expression tag
Pro -57	-	expression tag
Asn -56	-	expression tag
Gly -55	-	expression tag
Ser -54	-	expression tag
His -53	-	expression tag
Ala -52	-	expression tag
Pro -51	-	expression tag
Asp -50	-	expression tag
His -49	-	expression tag
Asn -48	-	expression tag
Val -47	-	expression tag
Thr -46	-	expression tag
Gln -45	-	expression tag
Gln -44	-	expression tag
Arg -43	-	expression tag
Asp -42	-	expression tag
Glu -41	-	expression tag
Gly -40	-	expression tag
Gly -39	-	expression tag
Ser -38	-	expression tag
Gly -37	-	expression tag
Gln -36	-	expression tag
Pro -35	-	expression tag
Gly -34	-	expression tag
Asn -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Ala -30	-	expression tag
Phe -29	-	expression tag
Leu -28	-	expression tag
Leu -27	-	expression tag
Ala -26	-	expression tag
Pro -25	-	expression tag
Asn -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
His -21	-	expression tag
Ala -20	-	expression tag
Pro -19	-	expression tag
Asp -18	-	expression tag
His -17	-	expression tag
Asn -16	-	expression tag
Val -15	-	expression tag
Thr -14	-	expression tag
Gln -13	-	expression tag
Gln -12	-	expression tag
Arg -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Val -1	-	expression tag
Asp 0	-	expression tag

R: 309 - 483 (PDB: 8YN3)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	155361.8
Non-Polymers*	Number of molecules	2
	Total formula weight	497.8
All*	Total formula weight	155859.6

*Water molecules are not included.