8YKY

Structure of human class T GPCR TAS2R14-Ggustducin complex with agonist 28.1

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	G alpha gustducin protein	polymer	369	42373.0	1	Pfam (PF00503) UniProt (by SIFTS) (A8MTJ3)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	366	39728.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (S)	ScFv16 protein	polymer	286	30668.2	1		Homo sapiens (human)
5	E (R)	exo-alpha-sialidase,Taste receptor type 2 member 14,LgBit	polymer	990	110840.0	1	UniProt (Q59310) UniProt (Q9NYV8) Pfam (PF13859) Pfam (PF05296)	Clostridium perfringens	T2R14,Taste receptor family B member 1,TRB1
6	F (R)	4-methyl-N-[(2M)-2-(1H-tetrazol-5-yl)phenyl]-6-(trifluoromethyl)pyrimidin-2-amine	non-polymer		321.3	1	Chemie (A1AEI)
7	G (R)	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)

Sequence modifications

B: 1 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: -455 - -4 (UniProt: Q59310)

PDB	External Database	Details
Met -499	-	initiating methionine
Lys -498	-	expression tag
Thr -497	-	expression tag
Ile -496	-	expression tag
Ile -495	-	expression tag
Ala -494	-	expression tag
Leu -493	-	expression tag
Ser -492	-	expression tag
Tyr -491	-	expression tag
Ile -490	-	expression tag
Phe -489	-	expression tag
Cys -488	-	expression tag
Leu -487	-	expression tag
Val -486	-	expression tag
Phe -485	-	expression tag
Ala -484	-	expression tag
Asp -483	-	expression tag
Tyr -482	-	expression tag
Lys -481	-	expression tag
Asp -480	-	expression tag
Asp -479	-	expression tag
Asp -478	-	expression tag
Asp -477	-	expression tag
Ala -476	-	expression tag
His -475	-	expression tag
His -474	-	expression tag
His -473	-	expression tag
His -472	-	expression tag
His -471	-	expression tag
His -470	-	expression tag
His -469	-	expression tag
His -468	-	expression tag
His -467	-	expression tag
His -466	-	expression tag
Glu -465	-	expression tag
Asn -464	-	expression tag
Leu -463	-	expression tag
Tyr -462	-	expression tag
Phe -461	-	expression tag
Gln -460	-	expression tag
Ser -459	-	expression tag
Gly -458	-	expression tag
Arg -457	-	expression tag
Ala -456	-	expression tag
Ser -305	Gly 393	conflict
Gly -3	-	linker
Ser -2	-	linker
Ala -1	-	linker
Gly 0	-	linker
Ser 1	-	linker

R: 2 - 317 (UniProt: Q9NYV8)
R: 318 - 490 (PDB: 8YKY)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	231470.8
Non-Polymers*	Number of molecules	2
	Total formula weight	707.9
All*	Total formula weight	232178.8

*Water molecules are not included.